Vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic stress

Nicole C.A. van Engeland, Freddy Suarez Rodriguez, Adolfo Rivero-Müller, Tommaso Ristori, Camille L. Duran, Oscar M.J.A. Stassen, Daniel Antfolk, Rob C.H. Driessen, Saku Ruohonen, Suvi T. Ruohonen, Salla Nuutinen, Eriika Savontaus, Sandra Loerakker, Kayla J. Bayless, Marika Sjöqvist, Carlijn V.C. Bouten, John E. Eriksson, Cecilia M. Sahlgren (Corresponding author)

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The intermediate filament (IF) cytoskeleton has been proposed to regulate morphogenic processes by integrating the cell fate signaling machinery with mechanical cues. Signaling between endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) through the Notch pathway regulates arterial remodeling in response to changes in blood flow. Here we show that the IF-protein vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic forces. Vimentin is important for Notch transactivation by ECs and vimentin knockout mice (VimKO) display disrupted VSMC differentiation and adverse remodeling in aortic explants and in vivo. Shear stress increases Jagged1 levels and Notch activation in a vimentin-dependent manner. Shear stress induces phosphorylation of vimentin at serine 38 and phosphorylated vimentin interacts with Jagged1 and increases Notch activation potential. Reduced Jagged1-Notch transactivation strength disrupts lateral signal induction through the arterial wall leading to adverse remodeling. Taken together we demonstrate that vimentin forms a central part of a mechanochemical transduction pathway that regulates multilayer communication and structural homeostasis of the arterial wall.

Originele taal-2Engels
Artikelnummer12415
Aantal pagina's14
TijdschriftScientific Reports
Volume9
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 27 aug 2019

Vingerafdruk

Vimentin
Hemodynamics
Vascular Smooth Muscle
Transcriptional Activation
Smooth Muscle Myocytes
Endothelial Cells
Intermediate Filament Proteins
Intermediate Filaments
Cytoskeleton
Knockout Mice
Serine
Cues
Cell Differentiation
Homeostasis
Communication
Phosphorylation

Citeer dit

van Engeland, Nicole C.A. ; Suarez Rodriguez, Freddy ; Rivero-Müller, Adolfo ; Ristori, Tommaso ; Duran, Camille L. ; Stassen, Oscar M.J.A. ; Antfolk, Daniel ; Driessen, Rob C.H. ; Ruohonen, Saku ; Ruohonen, Suvi T. ; Nuutinen, Salla ; Savontaus, Eriika ; Loerakker, Sandra ; Bayless, Kayla J. ; Sjöqvist, Marika ; Bouten, Carlijn V.C. ; Eriksson, John E. ; Sahlgren, Cecilia M. / Vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic stress. In: Scientific Reports. 2019 ; Vol. 9, Nr. 1.
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abstract = "The intermediate filament (IF) cytoskeleton has been proposed to regulate morphogenic processes by integrating the cell fate signaling machinery with mechanical cues. Signaling between endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) through the Notch pathway regulates arterial remodeling in response to changes in blood flow. Here we show that the IF-protein vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic forces. Vimentin is important for Notch transactivation by ECs and vimentin knockout mice (VimKO) display disrupted VSMC differentiation and adverse remodeling in aortic explants and in vivo. Shear stress increases Jagged1 levels and Notch activation in a vimentin-dependent manner. Shear stress induces phosphorylation of vimentin at serine 38 and phosphorylated vimentin interacts with Jagged1 and increases Notch activation potential. Reduced Jagged1-Notch transactivation strength disrupts lateral signal induction through the arterial wall leading to adverse remodeling. Taken together we demonstrate that vimentin forms a central part of a mechanochemical transduction pathway that regulates multilayer communication and structural homeostasis of the arterial wall.",
author = "{van Engeland}, {Nicole C.A.} and {Suarez Rodriguez}, Freddy and Adolfo Rivero-M{\"u}ller and Tommaso Ristori and Duran, {Camille L.} and Stassen, {Oscar M.J.A.} and Daniel Antfolk and Driessen, {Rob C.H.} and Saku Ruohonen and Ruohonen, {Suvi T.} and Salla Nuutinen and Eriika Savontaus and Sandra Loerakker and Bayless, {Kayla J.} and Marika Sj{\"o}qvist and Bouten, {Carlijn V.C.} and Eriksson, {John E.} and Sahlgren, {Cecilia M.}",
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van Engeland, NCA, Suarez Rodriguez, F, Rivero-Müller, A, Ristori, T, Duran, CL, Stassen, OMJA, Antfolk, D, Driessen, RCH, Ruohonen, S, Ruohonen, ST, Nuutinen, S, Savontaus, E, Loerakker, S, Bayless, KJ, Sjöqvist, M, Bouten, CVC, Eriksson, JE & Sahlgren, CM 2019, 'Vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic stress', Scientific Reports, vol. 9, nr. 1, 12415. https://doi.org/10.1038/s41598-019-48218-w

Vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic stress. / van Engeland, Nicole C.A.; Suarez Rodriguez, Freddy; Rivero-Müller, Adolfo; Ristori, Tommaso; Duran, Camille L.; Stassen, Oscar M.J.A.; Antfolk, Daniel; Driessen, Rob C.H.; Ruohonen, Saku; Ruohonen, Suvi T.; Nuutinen, Salla; Savontaus, Eriika; Loerakker, Sandra; Bayless, Kayla J.; Sjöqvist, Marika; Bouten, Carlijn V.C.; Eriksson, John E.; Sahlgren, Cecilia M. (Corresponding author).

In: Scientific Reports, Vol. 9, Nr. 1, 12415, 27.08.2019.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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AU - van Engeland, Nicole C.A.

AU - Suarez Rodriguez, Freddy

AU - Rivero-Müller, Adolfo

AU - Ristori, Tommaso

AU - Duran, Camille L.

AU - Stassen, Oscar M.J.A.

AU - Antfolk, Daniel

AU - Driessen, Rob C.H.

AU - Ruohonen, Saku

AU - Ruohonen, Suvi T.

AU - Nuutinen, Salla

AU - Savontaus, Eriika

AU - Loerakker, Sandra

AU - Bayless, Kayla J.

AU - Sjöqvist, Marika

AU - Bouten, Carlijn V.C.

AU - Eriksson, John E.

AU - Sahlgren, Cecilia M.

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N2 - The intermediate filament (IF) cytoskeleton has been proposed to regulate morphogenic processes by integrating the cell fate signaling machinery with mechanical cues. Signaling between endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) through the Notch pathway regulates arterial remodeling in response to changes in blood flow. Here we show that the IF-protein vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic forces. Vimentin is important for Notch transactivation by ECs and vimentin knockout mice (VimKO) display disrupted VSMC differentiation and adverse remodeling in aortic explants and in vivo. Shear stress increases Jagged1 levels and Notch activation in a vimentin-dependent manner. Shear stress induces phosphorylation of vimentin at serine 38 and phosphorylated vimentin interacts with Jagged1 and increases Notch activation potential. Reduced Jagged1-Notch transactivation strength disrupts lateral signal induction through the arterial wall leading to adverse remodeling. Taken together we demonstrate that vimentin forms a central part of a mechanochemical transduction pathway that regulates multilayer communication and structural homeostasis of the arterial wall.

AB - The intermediate filament (IF) cytoskeleton has been proposed to regulate morphogenic processes by integrating the cell fate signaling machinery with mechanical cues. Signaling between endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) through the Notch pathway regulates arterial remodeling in response to changes in blood flow. Here we show that the IF-protein vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic forces. Vimentin is important for Notch transactivation by ECs and vimentin knockout mice (VimKO) display disrupted VSMC differentiation and adverse remodeling in aortic explants and in vivo. Shear stress increases Jagged1 levels and Notch activation in a vimentin-dependent manner. Shear stress induces phosphorylation of vimentin at serine 38 and phosphorylated vimentin interacts with Jagged1 and increases Notch activation potential. Reduced Jagged1-Notch transactivation strength disrupts lateral signal induction through the arterial wall leading to adverse remodeling. Taken together we demonstrate that vimentin forms a central part of a mechanochemical transduction pathway that regulates multilayer communication and structural homeostasis of the arterial wall.

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