Samenvatting
The interaction of nanoparticles with biological media is a topic of general interest for drug delivery systems and among those for active nanoparticles, also called nanomotors. Herein, we report the use of super resolution microscopy, in particular, stochastic optical reconstruction microscopy (STORM), to characterize the formation of a protein corona around active enzyme-powered nanomotors. First, we characterized the distribution and number of enzymes on nano-sized particles and characterized their motion capabilities. Then, we incubated the nanomotors with fluorescently labelled serum proteins. Interestingly, we observed a significant decrease of protein corona formation (20%) and different composition, which was studied by proteomic analysis. Moreover, motion was not hindered, as nanomotors displayed enhanced diffusion regardless of the protein corona. Elucidating how active particles interact with biological media and maintain their self-propulsion after protein corona formation will pave the way for the use of these systems in complex biological fluids in biomedicine.
| Originele taal-2 | Engels |
|---|---|
| Pagina's (van-tot) | 2904-2912 |
| Aantal pagina's | 9 |
| Tijdschrift | Nanoscale |
| Volume | 16 |
| Nummer van het tijdschrift | 6 |
| Vroegere onlinedatum | 6 dec. 2023 |
| DOI's | |
| Status | Gepubliceerd - 14 feb. 2024 |
Financiering
This work was supported by the European Union's Horizon 2020 Research and Innovation Program, under the MarieSkłodowska-Curie Individual Fellowship (843998 to TP), and the Irène Curie Fellowship (TP). S. S. thanks the European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation Programme (grant agreement no 866348, i-NanoSwarms), PID2021-128417OB-I00 and PDC2022-133753-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” and European Union Next Generation EU (Bots4BB and BOJOS projects). S. S. thanks the “La Caixa” Foundation under the grant agreement LCF/PR/HR21/52410022 for project “BLADDEBOTS”. S. P. and L.A acknowledge the financial support by the Spanish Ministry of Science and Innovation (PID2019-109450RB-I00/AEI /10.13039/501100011033), European Research Council/Horizon 2020 (ERC-StG-757397), and the “la Caixa” Foundation (ID 100010434). Mass spectrometry/Proteomics was performed at the IRB Barcelona Mass Spectrometry and Proteomics Core Facility, which is a member of ProteoRed, PRB3-ISCIII, supported by grant PRB3 (IPT17/0019 – ISCIII-SGEFI/ERDF) and is granted in the framework of the 2014–2020 ERDF Operational Programme in Catalonia, co-financed by the European Regional Development Fund (ERDF, Reference: IU16-015983). The IBEC team thanks the CERCA program by the Generalitat de Catalunya, the Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement de la Generalitat de Catalunya through the project 2021 SGR 01606, and the “Centro de Excelencia Severo Ochoa”, funded by Agencia Estatal de Investigación (CEX2018-000789-S).
| Financiers | Financiernummer |
|---|---|
| Centro de Excelencia Severo Ochoa | |
| European Union’s Horizon Europe research and innovation programme | ERC-StG-757397 |
| European Union Next Generation EU/PRTR | LCF/PR/HR21/52410022 |
| IRB Barcelona Mass Spectrometry and Proteomics Core Facility | PRB3-ISCIII, IPT17/0019 – ISCIII-SGEFI/ERDF |
| European Research Council | |
| Generalitat de Catalunya | 2021 SGR 01606 |
| Ministerio de Ciencia e Innovación | PID2019-109450RB-I00/AEI /10.13039/501100011033 |
| Horizon 2020 | MCIN/AEI/10.13039/501100011033, 843998, PID2021-128417OB-I00, PDC2022-133753-I00, 866348 |
| European Regional Development Fund | IU16-015983 |
| Agencia Estatal de Investigación | CEX2018-000789-S |
| “la Caixa” Foundation | 100010434 |