Unravelling mechanisms of protein and lipid oxidation in mayonnaise at multiple length scales

Suyeon Yang, Machi Takeuchi, Heiner Friedrich (Corresponding author), John P.M. van Duynhoven (Corresponding author), Johannes Hohlbein (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

8 Citaten (Scopus)
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Samenvatting

In mayonnaise, lipid and protein oxidation are closely related and the interplay between them is critical for understanding the chemical shelf-life stability of mayonnaise. This is in particular the case for comprehending the role of low-density lipoprotein (LDL) particles acting as a main emulsifier. Here, we monitored oxidation and the concomitant aggregation of LDLs by bright-field light microscopy and cryogenic transmission electron microscopy. We further probed the formation of protein radicals and protein oxidation by imaging the accumulation of a water-soluble fluorescent spin trap and protein autofluorescence. The effect of variation of pH and addition of EDTA on the accumulation of the spin trap validated that protein radicals were induced by lipid radicals. Our data suggests two main pathways of oxidative protein radical formation in LDL particles: (1) at the droplet interface, induced by lipid free radicals formed in oil droplets, and (2) in the continuous phase induced by an independent LDL-specific mechanism.

Originele taal-2Engels
Artikelnummer134417
Aantal pagina's9
TijdschriftFood Chemistry
Volume402
DOI's
StatusGepubliceerd - 15 feb. 2023

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© 2022 The Author(s)

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