Tuning the metal binding site specificity of a fluorescent sensor protein: from copper to zinc and back

M.S.T. Koay, B.M.G. Janssen, M. Merkx

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

17 Citaten (Scopus)
1 Downloads (Pure)

Samenvatting

We previously reported the development of high affinity Zn2+ FRET sensors based on the Zn2+-mediated interaction between the CXXC motifs present in the copper chaperone proteins ATOX1 and WD4. By systematically substituting several of these cysteines for methionines, we constructed sensor variants that retain a high affinity for Cu+, while effectively abolishing their ability to form stable tetrahedral Zn2+ complexes.
Originele taal-2Engels
Pagina's (van-tot)3230-3232
Aantal pagina's3
TijdschriftDalton Transactions
Volume42
DOI's
StatusGepubliceerd - 2013

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