Tough magnesium phosphate-based 3D-printed implants induce bone regeneration in an equine defect model

Nasim Golafshan, Elke Vorndran, Stefan Zaharievski, Harold Brommer, Firoz Babu Kadumudi, Alireza Dolatshahi-Pirouz, Uwe Gbureck, René van Weeren, Miguel Castilho (Corresponding author), Jos Malda

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

91 Citaten (Scopus)


One of the important challenges in bone tissue engineering is the development of biodegradable bone substitutes with appropriate mechanical and biological properties for the treatment of larger defects and those with complex shapes. Recently, magnesium phosphate (MgP) doped with biologically active ions like strontium (Sr2+) have shown to significantly enhance bone formation when compared with the standard calcium phosphate-based ceramics. However, such materials can hardly be shaped into large and complex geometries and more importantly lack the adequate mechanical properties for the treatment of load-bearing bone defects. In this study, we have fabricated bone implants through extrusion assisted three-dimensional (3D) printing of MgP ceramics modified with Sr2+ ions (MgPSr) and a medical-grade polycaprolactone (PCL) polymer phase. MgPSr with 30 wt% PCL (MgPSr-PCL30) allowed the printability of relevant size structures (>780 mm3) at room temperature with an interconnected macroporosity of approximately 40%. The printing resulted in implants with a compressive strength of 4.3 MPa, which were able to support up to 50 cycles of loading without plastic deformation. Notably, MgPSr-PCL30 scaffolds were able to promote in vitro bone formation in medium without the supplementation with osteo-inducing components. In addition, long-term in vivo performance of the 3D printed scaffolds was investigated in an equine tuber coxae model over 6 months. The micro-CT and histological analysis showed that implantation of MgPSr-PCL30 induced bone regeneration, while no bone formation was observed in the empty defects. Overall, the novel polymer-modified MgP ceramic material and extrusion-based 3D printing process presented here greatly improved the shape ability and load-bearing properties of MgP-based ceramics with simultaneous induction of new bone formation.

Originele taal-2Engels
Aantal pagina's11
StatusGepubliceerd - dec. 2020


The research leading to these results has received financial support from the European Research Council under grant agreement 647426 (3D-JOINT), the Dutch Arthritis Foundation ( LLP-12 and LLP-22 ), the strategic alliance University Medical Center Utrecht – Technical University Eindhoven, and the partners of Regenerative Medicine Crossing Borders ( ) powered by Health~Holland, Top Sector Life Sciences & Health . In addition, we gratefully thank Saskia Plomp, Mattie van Rijen, and Inge Dokter for excellent histology technical assistance and Dr. Jacqueline Alblas and Dr. Maaike Braham for useful discussions and support with in vitro experiments.

Dutch Arthritis FoundationLLP-22, LLP-12
Regenerative Medicine Crossing Borders
UMC Utrecht
Horizon 2020 Framework Programme647426
European Research Council3D-JOINT


    Duik in de onderzoeksthema's van 'Tough magnesium phosphate-based 3D-printed implants induce bone regeneration in an equine defect model'. Samen vormen ze een unieke vingerafdruk.

    Citeer dit