The effect of salts upon the pH dependence of the activity of papain and succinyl-papain

L.A.A.E. Sluyterman; M.J.M. Graaf, de

doi:10.1016/0005-2744(72)90247-1

The effect of salts upon the pH dependence of the activity of papain and succinyl-papain

L.A.A.E. Sluyterman, M.J.M. Graaf, de

Chemical Engineering and Chemistry

Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review

36 Citaten (Scopus)

Samenvatting

Papain (EC 3.4.4.10) and succinyl-papain exhibit isoelectric points of 9.6 and 4.3, respectively. The acid limb of the pH activity curve in the pH range of 3.5–4.5 of native papain and the basic limb in the pH range of 8–9 of succinyl-papain are shifted by monovalent salts, whereas the basic limb of native papain and the acid limb of succinyl-papain are not shifted by KCl. The Km and not the kcat with benzoylglycine ethyl ester as substrate is affected by salt. The order of the effectiveness of the ions is I- > NO3- > Br- > Cl- and Li+ > K+. These effects can be explained in terms of non-specific binding of cations and anions and electrostatic interactions.

Originele taal-2	Engels
Pagina's (van-tot)	554-561
Aantal pagina's	8
Tijdschrift	Biochimica et Biophysica Acta, Enzymology
Volume	258
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1016/0005-2744(72)90247-1
Status	Gepubliceerd - 1972

Toegang tot document

10.1016/0005-2744(72)90247-1

Citeer dit

@article{642c7d3d482e4fc1ba58486d07a76088,

title = "The effect of salts upon the pH dependence of the activity of papain and succinyl-papain",

abstract = "Papain (EC 3.4.4.10) and succinyl-papain exhibit isoelectric points of 9.6 and 4.3, respectively. The acid limb of the pH activity curve in the pH range of 3.5–4.5 of native papain and the basic limb in the pH range of 8–9 of succinyl-papain are shifted by monovalent salts, whereas the basic limb of native papain and the acid limb of succinyl-papain are not shifted by KCl. The Km and not the kcat with benzoylglycine ethyl ester as substrate is affected by salt. The order of the effectiveness of the ions is I- > NO3- > Br- > Cl- and Li+ > K+. These effects can be explained in terms of non-specific binding of cations and anions and electrostatic interactions.",

author = "L.A.A.E. Sluyterman and {Graaf, de}, M.J.M.",

year = "1972",

doi = "10.1016/0005-2744(72)90247-1",

language = "English",

volume = "258",

pages = "554--561",

journal = "Biochimica et Biophysica Acta, Enzymology",

issn = "0924-1086",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - The effect of salts upon the pH dependence of the activity of papain and succinyl-papain

AU - Sluyterman, L.A.A.E.

AU - Graaf, de, M.J.M.

PY - 1972

Y1 - 1972

N2 - Papain (EC 3.4.4.10) and succinyl-papain exhibit isoelectric points of 9.6 and 4.3, respectively. The acid limb of the pH activity curve in the pH range of 3.5–4.5 of native papain and the basic limb in the pH range of 8–9 of succinyl-papain are shifted by monovalent salts, whereas the basic limb of native papain and the acid limb of succinyl-papain are not shifted by KCl. The Km and not the kcat with benzoylglycine ethyl ester as substrate is affected by salt. The order of the effectiveness of the ions is I- > NO3- > Br- > Cl- and Li+ > K+. These effects can be explained in terms of non-specific binding of cations and anions and electrostatic interactions.

AB - Papain (EC 3.4.4.10) and succinyl-papain exhibit isoelectric points of 9.6 and 4.3, respectively. The acid limb of the pH activity curve in the pH range of 3.5–4.5 of native papain and the basic limb in the pH range of 8–9 of succinyl-papain are shifted by monovalent salts, whereas the basic limb of native papain and the acid limb of succinyl-papain are not shifted by KCl. The Km and not the kcat with benzoylglycine ethyl ester as substrate is affected by salt. The order of the effectiveness of the ions is I- > NO3- > Br- > Cl- and Li+ > K+. These effects can be explained in terms of non-specific binding of cations and anions and electrostatic interactions.

U2 - 10.1016/0005-2744(72)90247-1

DO - 10.1016/0005-2744(72)90247-1

M3 - Article

SN - 0924-1086

VL - 258

SP - 554

EP - 561

JO - Biochimica et Biophysica Acta, Enzymology

JF - Biochimica et Biophysica Acta, Enzymology

IS - 2

ER -

The effect of salts upon the pH dependence of the activity of papain and succinyl-papain

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit