The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

E.A. Bruijn, de; P.A. Leclercq; U.R. Tjaden

The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

E.A. Bruijn, de
, P.A. Leclercq
, U.R. Tjaden

Chemical Engineering and Chemistry

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Conferentiebijdrage › Academic › peer review

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The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.

Originele taal-2	Engels
Titel	Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985
Redacteuren	P. Sandra, W. Bertsch
Plaats van productie	Heidelberg
Uitgeverij	Hüthig
Pagina's	599-608
Status	Gepubliceerd - 1985

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title = "The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography",

abstract = "The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10\%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.",

author = "\{Bruijn, de\}, E.A. and P.A. Leclercq and U.R. Tjaden",

year = "1985",

language = "English",

pages = "599--608",

editor = "P. Sandra and W. Bertsch",

booktitle = "Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985",

publisher = "H{\"u}thig",

address = "Germany",

}

The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography. / Bruijn, de, E.A.; Leclercq, P.A.; Tjaden, U.R.
Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985. reds. / P. Sandra; W. Bertsch. Heidelberg: Hüthig, 1985. blz. 599-608.

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Conferentiebijdrage › Academic › peer review

TY - GEN

T1 - The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

AU - Bruijn, de, E.A.

AU - Leclercq, P.A.

AU - Tjaden, U.R.

PY - 1985

Y1 - 1985

N2 - The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.

AB - The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.

M3 - Conference contribution

SP - 599

EP - 608

BT - Proceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985

A2 - Sandra, P.

A2 - Bertsch, W.

PB - Hüthig

CY - Heidelberg

ER -

The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

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