The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography

E.A. Bruijn, de, P.A. Leclercq, U.R. Tjaden

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureConferentiebijdrageAcademicpeer review

Samenvatting

The selectivity of a capillary gas chromatographic assay for the antineoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-keto cyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies revealed that a cyclization product of 4-keto CPA can be formed during the same chromatographic conditions under which CPA has been determined. However, the produced amount is relatively small (less than 10%) in comparison with the percentage produced from CPA. Furthermore, both cyclization products, formed by a loss of HCI and intra alkylation of the parent compounds, can be well separated under proper chromatographic conditions.
Originele taal-2Engels
TitelProceedings of the Sixth International symposium on capillary chromatography, Riva del Garda, 14-16 May 1985
RedacteurenP. Sandra, W. Bertsch
Plaats van productieHeidelberg
UitgeverijHuethig
Pagina's599-608
StatusGepubliceerd - 1985

Vingerafdruk

Duik in de onderzoeksthema's van 'The autoderivatization of cyclophosphamide and its metabolite 4-keto cyclophosphamide during capillary gas chromatography'. Samen vormen ze een unieke vingerafdruk.

Citeer dit