Temperature-controlled positioning of fusion proteins in microreactors

R.L.M. Teeuwen; H. Zuilhof; F.A. de Wolf; J.C.M. van Hest

doi:10.1039/b903087e

Temperature-controlled positioning of fusion proteins in microreactors

R.L.M. Teeuwen
, H. Zuilhof
, F.A. de Wolf
, J.C.M. van Hest

Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review

12 Citaten (Scopus)

Samenvatting

We present a non-covalent immobilization system based on stimulus-responsive elastin-like polypeptides (ELPs) to facilitate the positioning of proteins in microchannels. Two ELP variants were constructed and connected to fluorescent proteins EGFP and DsRed2. These fusion proteins display an inverse transition behavior that can be simply controlled by varying concentrations of NaCl. With these ELP fusion proteins two patches of fluorescent proteins can be formed inside a microreactor, using only the temperature-responsive property of ELPs.

Originele taal-2	Engels
Pagina's (van-tot)	2261-2268
Aantal pagina's	8
Tijdschrift	Soft Matter
Volume	5
Nummer van het tijdschrift	11
DOI's	https://doi.org/10.1039/b903087e
Status	Gepubliceerd - 2009
Extern gepubliceerd	Ja

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abstract = "We present a non-covalent immobilization system based on stimulus-responsive elastin-like polypeptides (ELPs) to facilitate the positioning of proteins in microchannels. Two ELP variants were constructed and connected to fluorescent proteins EGFP and DsRed2. These fusion proteins display an inverse transition behavior that can be simply controlled by varying concentrations of NaCl. With these ELP fusion proteins two patches of fluorescent proteins can be formed inside a microreactor, using only the temperature-responsive property of ELPs.",

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AU - de Wolf, F.A.

AU - van Hest, J.C.M.

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AB - We present a non-covalent immobilization system based on stimulus-responsive elastin-like polypeptides (ELPs) to facilitate the positioning of proteins in microchannels. Two ELP variants were constructed and connected to fluorescent proteins EGFP and DsRed2. These fusion proteins display an inverse transition behavior that can be simply controlled by varying concentrations of NaCl. With these ELP fusion proteins two patches of fluorescent proteins can be formed inside a microreactor, using only the temperature-responsive property of ELPs.

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Temperature-controlled positioning of fusion proteins in microreactors

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