Structure and functional analysis of a bacterial adhesin sugar-binding domain

Tyler D.R. Vance, Shuaiqi Guo, Shayan Assaie-Ardakany, Brigid Conroy, Peter L. Davies (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

Uittreksel

Bacterial adhesins attach their hosts to surfaces through one or more ligand-binding domains. In RTX adhesins, which are localized to the outer membrane of many Gram-negative bacteria via the type I secretion system, we see several examples of a putative sugar-binding domain. Here we have recombinantly expressed one such ~20-kDa domain from the ~340-kDa adhesin found in Marinobacter hydrocarbonoclasticus, an oil-degrading bacterium. The sugar-binding domain was purified from E. coli with a yield of 100 mg/L of culture. Circular dichroism analysis showed that the protein was rich in beta-structure, was moderately heat resistant, and required Ca2+ for proper folding. A crystal structure was obtained in Ca2+ at 1.2-Å resolution, which showed the presence of three Ca2+ ions, two of which were needed for structural integrity and one for binding sugars. Glucose was soaked into the crystal, where it bound to the sugar’s two vicinal hydroxyl groups attached to the first and second (C1 and C2) carbons in the pyranose ring. This attraction to glucose caused the protein to bind certain polysaccharide-based column matrices and was used in a simple competitive binding assay to assess the relative affinity of sugars for the protein’s ligand-binding site. Fucose, glucose and N-acetylglucosamine bound most tightly, and N-acetylgalactosamine hardly bound at all. Isothermal titration calorimetry was used to determine specific binding affinities, which lie in the 100-μM range. Glycan arrays were tested to expand the range of ligand sugars assayed, and showed that MhPA14 bound preferentially to branched polymers containing terminal sugars highlighted as strong binders in the competitive binding assay. Some of these binders have vicinal hydroxyl groups attached to the C3 and C4 carbons that are sterically equivalent to those presented by the C1 and C2 carbons of glucose.

TaalEngels
Artikelnummere0220045
Aantal pagina's26
TijdschriftPLoS ONE
Volume14
Nummer van het tijdschrift7
DOI's
StatusGepubliceerd - 23 jul 2019

Vingerafdruk

Bacterial Adhesins
carbohydrate binding
Functional analysis
adhesins
Sugars
sugars
Glucose
glucose
Competitive Binding
Carbon
Ligands
Hydroxyl Radical
Polysaccharides
carbon
Marinobacter hydrocarbonoclasticus
Marinobacter
polysaccharides
circular dichroism spectroscopy
Acetylgalactosamine
N-acetylglucosamine

Citeer dit

Vance, T. D. R., Guo, S., Assaie-Ardakany, S., Conroy, B., & Davies, P. L. (2019). Structure and functional analysis of a bacterial adhesin sugar-binding domain. PLoS ONE, 14(7), [e0220045]. DOI: 10.1371/journal.pone.0220045
Vance, Tyler D.R. ; Guo, Shuaiqi ; Assaie-Ardakany, Shayan ; Conroy, Brigid ; Davies, Peter L./ Structure and functional analysis of a bacterial adhesin sugar-binding domain. In: PLoS ONE. 2019 ; Vol. 14, Nr. 7.
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abstract = "Bacterial adhesins attach their hosts to surfaces through one or more ligand-binding domains. In RTX adhesins, which are localized to the outer membrane of many Gram-negative bacteria via the type I secretion system, we see several examples of a putative sugar-binding domain. Here we have recombinantly expressed one such ~20-kDa domain from the ~340-kDa adhesin found in Marinobacter hydrocarbonoclasticus, an oil-degrading bacterium. The sugar-binding domain was purified from E. coli with a yield of 100 mg/L of culture. Circular dichroism analysis showed that the protein was rich in beta-structure, was moderately heat resistant, and required Ca2+ for proper folding. A crystal structure was obtained in Ca2+ at 1.2-{\AA} resolution, which showed the presence of three Ca2+ ions, two of which were needed for structural integrity and one for binding sugars. Glucose was soaked into the crystal, where it bound to the sugar’s two vicinal hydroxyl groups attached to the first and second (C1 and C2) carbons in the pyranose ring. This attraction to glucose caused the protein to bind certain polysaccharide-based column matrices and was used in a simple competitive binding assay to assess the relative affinity of sugars for the protein’s ligand-binding site. Fucose, glucose and N-acetylglucosamine bound most tightly, and N-acetylgalactosamine hardly bound at all. Isothermal titration calorimetry was used to determine specific binding affinities, which lie in the 100-μM range. Glycan arrays were tested to expand the range of ligand sugars assayed, and showed that MhPA14 bound preferentially to branched polymers containing terminal sugars highlighted as strong binders in the competitive binding assay. Some of these binders have vicinal hydroxyl groups attached to the C3 and C4 carbons that are sterically equivalent to those presented by the C1 and C2 carbons of glucose.",
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Vance, TDR, Guo, S, Assaie-Ardakany, S, Conroy, B & Davies, PL 2019, 'Structure and functional analysis of a bacterial adhesin sugar-binding domain' PLoS ONE, vol. 14, nr. 7, e0220045. DOI: 10.1371/journal.pone.0220045

Structure and functional analysis of a bacterial adhesin sugar-binding domain. / Vance, Tyler D.R.; Guo, Shuaiqi; Assaie-Ardakany, Shayan; Conroy, Brigid; Davies, Peter L. (Corresponding author).

In: PLoS ONE, Vol. 14, Nr. 7, e0220045, 23.07.2019.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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Vance TDR, Guo S, Assaie-Ardakany S, Conroy B, Davies PL. Structure and functional analysis of a bacterial adhesin sugar-binding domain. PLoS ONE. 2019 jul 23;14(7). e0220045. Beschikbaar vanaf, DOI: 10.1371/journal.pone.0220045