Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells

Arianna Mauretti; Fabrizio Rossi; Noortje A.M. Bax; Carmen Miano; Fabio Miraldi; Marie José Goumans; Elisa Messina; Alessandro Giacomello; Carlijn V.C. Bouten; Cecilia Sahlgren

doi:10.1557/mrc.2017.82

Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells

Arianna Mauretti, Fabrizio Rossi, Noortje A.M. Bax, Carmen Miano, Fabio Miraldi, Marie José Goumans, Elisa Messina, Alessandro Giacomello, Carlijn V.C. Bouten, Cecilia Sahlgren

Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review

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Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O₂ further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.

Originele taal-2	Engels
Pagina's (van-tot)	496-501
Aantal pagina's	6
Tijdschrift	MRS Communications
Volume	7
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1557/mrc.2017.82
Status	Gepubliceerd - 1 sep. 2017

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title = "Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells",

abstract = "Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.",

author = "Arianna Mauretti and Fabrizio Rossi and Bax, {Noortje A.M.} and Carmen Miano and Fabio Miraldi and Goumans, {Marie Jos{\'e}} and Elisa Messina and Alessandro Giacomello and Bouten, {Carlijn V.C.} and Cecilia Sahlgren",

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T1 - Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells

AU - Mauretti, Arianna

AU - Rossi, Fabrizio

AU - Bax, Noortje A.M.

AU - Miano, Carmen

AU - Miraldi, Fabio

AU - Goumans, Marie José

AU - Messina, Elisa

AU - Giacomello, Alessandro

AU - Bouten, Carlijn V.C.

AU - Sahlgren, Cecilia

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.

AB - Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.

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DO - 10.1557/mrc.2017.82

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Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells

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