Spheroid culture as a tool for creating 3D complex tissues

Eelco Fennema, Nicolas Rivron, Jeroen Rouwkema, Clemens van Blitterswijk, Jan De Boer

Onderzoeksoutput: Bijdrage aan tijdschriftArtikel recenserenpeer review

769 Citaten (Scopus)


3D cell culture methods confer a high degree of clinical and biological relevance to in vitro models. This is specifically the case with the spheroid culture, where a small aggregate of cells grows free of foreign materials. In spheroid cultures, cells secrete the extracellular matrix (ECM) in which they reside, and they can interact with cells from their original microenvironment. The value of spheroid cultures is increasing quickly due to novel microfabricated platforms amenable to high-throughput screening (HTS) and advances in cell culture. Here, we review new possibilities that combine the strengths of spheroid culture with new microenvironment fabrication methods that allow for the creation of large numbers of highly reproducible, complex tissues.

Originele taal-2Engels
Pagina's (van-tot)108-115
Aantal pagina's8
TijdschriftTrends in Biotechnology
Nummer van het tijdschrift2
StatusGepubliceerd - 1 feb. 2013
Extern gepubliceerdJa


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