Single-step bioluminescence lateral flow immunoassays for diagnostics

Riho Shimazu; Junnosuke Kawahara; Kosuke Tomimuro; Kazushi Misawa; Yan Ni; Yuki Hiruta; Maarten Merkx; Daniel Citterio

Single-step bioluminescence lateral flow immunoassays for diagnostics

Riho Shimazu, Junnosuke Kawahara, Kosuke Tomimuro, Kazushi Misawa, Yan Ni, Yuki Hiruta, Maarten Merkx, Daniel Citterio (Corresponderende auteur)

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Conferentiebijdrage › Academic › peer review

Samenvatting

We present two diagnostic platforms, a microfluidic thread-based and a conventional immunochromatographic strip approach, for single-step lateral flow immunoassays (LFIAs) with bioluminescence (BL) signaling requiring only sample application and detection of emission color changes by a digital camera. Both rely on bioluminescence resonance energy transfer (BRET)-switching proteins. The detection of three clinically relevant targets is demonstrated: Cetuximab as example of an antibody-based anticancer drug requiring monitoring of blood drug levels in patients, and HIV1-p17 antibodies and antigens as examples of infectious disease markers.

Originele taal-2	Engels
Titel	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Uitgeverij	Chemical and Biological Microsystems Society
Pagina's	703-704
Aantal pagina's	2
ISBN van elektronische versie	9781733419000
Status	Gepubliceerd - okt. 2019
Evenement	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Zwitserland Duur: 27 okt. 2019 → 31 okt. 2019 https://microtas2019.org/

Congres

Congres	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Verkorte titel	MicroTAS 2019
Land/Regio	Zwitserland
Stad	Basel
Periode	27/10/19 → 31/10/19
Internet adres	https://microtas2019.org/

Bibliografische nota

Publisher Copyright:
© 2019 CBMS-0001.

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@inproceedings{f1136f2d4b604a13b9a2a49882fe003f,

title = "Single-step bioluminescence lateral flow immunoassays for diagnostics",

abstract = "We present two diagnostic platforms, a microfluidic thread-based and a conventional immunochromatographic strip approach, for single-step lateral flow immunoassays (LFIAs) with bioluminescence (BL) signaling requiring only sample application and detection of emission color changes by a digital camera. Both rely on bioluminescence resonance energy transfer (BRET)-switching proteins. The detection of three clinically relevant targets is demonstrated: Cetuximab as example of an antibody-based anticancer drug requiring monitoring of blood drug levels in patients, and HIV1-p17 antibodies and antigens as examples of infectious disease markers.",

keywords = "Bioluminescence, Blood analysis, BRET, Therapeutic drug monitoring, Thread-based devices",

author = "Riho Shimazu and Junnosuke Kawahara and Kosuke Tomimuro and Kazushi Misawa and Yan Ni and Yuki Hiruta and Maarten Merkx and Daniel Citterio",

note = "Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

month = oct,

language = "English",

pages = "703--704",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

publisher = "Chemical and Biological Microsystems Society",

url = "https://microtas2019.org/",

}

Shimazu, R, Kawahara, J, Tomimuro, K, Misawa, K, Ni, Y, Hiruta, Y, Merkx, M & Citterio, D 2019, Single-step bioluminescence lateral flow immunoassays for diagnostics. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, blz. 703-704, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Basel, Zwitserland, 27/10/19.

Single-step bioluminescence lateral flow immunoassays for diagnostics. / Shimazu, Riho; Kawahara, Junnosuke; Tomimuro, Kosuke et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. blz. 703-704.

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Conferentiebijdrage › Academic › peer review

TY - GEN

T1 - Single-step bioluminescence lateral flow immunoassays for diagnostics

AU - Shimazu, Riho

AU - Kawahara, Junnosuke

AU - Tomimuro, Kosuke

AU - Misawa, Kazushi

AU - Ni, Yan

AU - Hiruta, Yuki

AU - Merkx, Maarten

AU - Citterio, Daniel

PY - 2019/10

Y1 - 2019/10

N2 - We present two diagnostic platforms, a microfluidic thread-based and a conventional immunochromatographic strip approach, for single-step lateral flow immunoassays (LFIAs) with bioluminescence (BL) signaling requiring only sample application and detection of emission color changes by a digital camera. Both rely on bioluminescence resonance energy transfer (BRET)-switching proteins. The detection of three clinically relevant targets is demonstrated: Cetuximab as example of an antibody-based anticancer drug requiring monitoring of blood drug levels in patients, and HIV1-p17 antibodies and antigens as examples of infectious disease markers.

AB - We present two diagnostic platforms, a microfluidic thread-based and a conventional immunochromatographic strip approach, for single-step lateral flow immunoassays (LFIAs) with bioluminescence (BL) signaling requiring only sample application and detection of emission color changes by a digital camera. Both rely on bioluminescence resonance energy transfer (BRET)-switching proteins. The detection of three clinically relevant targets is demonstrated: Cetuximab as example of an antibody-based anticancer drug requiring monitoring of blood drug levels in patients, and HIV1-p17 antibodies and antigens as examples of infectious disease markers.

KW - Bioluminescence

KW - Blood analysis

KW - BRET

KW - Therapeutic drug monitoring

KW - Thread-based devices

UR - https://www.scopus.com/pages/publications/85094966640

M3 - Conference contribution

AN - SCOPUS:85094966640

SP - 703

EP - 704

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

PB - Chemical and Biological Microsystems Society

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Y2 - 27 October 2019 through 31 October 2019

ER -

Single-step bioluminescence lateral flow immunoassays for diagnostics

Samenvatting

Congres

Bibliografische nota

Duurzame ontwikkelingsdoelstellingen van de VN

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