TY - JOUR
T1 - Single-Molecule Optical Biosensing
T2 - Recent Advances and Future Challenges
AU - Dey, Swayandipta
AU - Dolci, Mathias
AU - Zijlstra, Peter
N1 - Funding Information:
This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 864772). This publication is part of the project “scalable lab-on-fiber optical sensing” (with project number 18477) which is financed by the Dutch Research Council (NWO).
PY - 2023/3/22
Y1 - 2023/3/22
N2 - In recent years, the sensitivity and specificity of optical sensors has improved tremendously due to improvements in biochemical functionalization protocols and optical detection systems. As a result, single-molecule sensitivity has been reported in a range of biosensing assay formats. In this Perspective, we summarize optical sensors that achieve single-molecule sensitivity in direct label-free assays, sandwich assays, and competitive assays. We describe the advantages and disadvantages of single-molecule assays and summarize future challenges in the field including their optical miniaturization and integration, multimodal sensing capabilities, accessible time scales, and compatibility with real-life matrices such as biological fluids. We conclude by highlighting the possible application areas of optical single-molecule sensors that include not only healthcare but also the monitoring of the environment and industrial processes.
AB - In recent years, the sensitivity and specificity of optical sensors has improved tremendously due to improvements in biochemical functionalization protocols and optical detection systems. As a result, single-molecule sensitivity has been reported in a range of biosensing assay formats. In this Perspective, we summarize optical sensors that achieve single-molecule sensitivity in direct label-free assays, sandwich assays, and competitive assays. We describe the advantages and disadvantages of single-molecule assays and summarize future challenges in the field including their optical miniaturization and integration, multimodal sensing capabilities, accessible time scales, and compatibility with real-life matrices such as biological fluids. We conclude by highlighting the possible application areas of optical single-molecule sensors that include not only healthcare but also the monitoring of the environment and industrial processes.
KW - binding kinetics
KW - continuous monitoring
KW - fluorescence
KW - multimodal sensors
KW - nanoparticles
KW - Single-molecule sensing
UR - http://www.scopus.com/inward/record.url?scp=85146330742&partnerID=8YFLogxK
U2 - 10.1021/acsphyschemau.2c00061
DO - 10.1021/acsphyschemau.2c00061
M3 - Review article
C2 - 36968450
AN - SCOPUS:85146330742
SN - 2694-2445
VL - 3
SP - 143
EP - 156
JO - ACS Physical Chemistry Au
JF - ACS Physical Chemistry Au
IS - 2
ER -