Uittreksel
In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.
| Originele taal-2 | Engels |
|---|---|
| Pagina's (van-tot) | 1-7 |
| Aantal pagina's | 7 |
| Tijdschrift | Microelectronic Engineering |
| Volume | 197 |
| DOI's | |
| Status | Gepubliceerd - 5 okt 2018 |
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Single cell trapping by capillary pumping using NOA81 replica moulded stencils. / Moonen, Emma; Luttge, Regina; Frimat, Jean Philippe.
In: Microelectronic Engineering, Vol. 197, 05.10.2018, blz. 1-7.Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review
TY - JOUR
T1 - Single cell trapping by capillary pumping using NOA81 replica moulded stencils
AU - Moonen, Emma
AU - Luttge, Regina
AU - Frimat, Jean Philippe
PY - 2018/10/5
Y1 - 2018/10/5
N2 - In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.
AB - In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.
KW - Brain-on-a-chip
KW - NOA81
KW - Passive pumping
KW - Replica moulding
KW - Single cell analysis
UR - http://www.scopus.com/inward/record.url?scp=85047100300&partnerID=8YFLogxK
U2 - 10.1016/j.mee.2018.04.010
DO - 10.1016/j.mee.2018.04.010
M3 - Article
AN - SCOPUS:85047100300
VL - 197
SP - 1
EP - 7
JO - Microelectronic Engineering
JF - Microelectronic Engineering
SN - 0167-9317
ER -