Single cell trapping by capillary pumping using NOA81 replica moulded stencils

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Uittreksel

In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.

Originele taal-2Engels
Pagina's (van-tot)1-7
Aantal pagina's7
TijdschriftMicroelectronic Engineering
Volume197
DOI's
StatusGepubliceerd - 5 okt 2018

Vingerafdruk

Syringes
Micromachining
replicas
Molding
Adhesives
pumping
trapping
Cells
Pumps
Fabrication
Lasers
cells
syringes
laser machining
micromachining
adhesives
pumps
fabrication
products

Citeer dit

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abstract = "In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80{\%} with high cell survival rates (90{\%} over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.",
keywords = "Brain-on-a-chip, NOA81, Passive pumping, Replica moulding, Single cell analysis",
author = "Emma Moonen and Regina Luttge and Frimat, {Jean Philippe}",
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Single cell trapping by capillary pumping using NOA81 replica moulded stencils. / Moonen, Emma; Luttge, Regina; Frimat, Jean Philippe.

In: Microelectronic Engineering, Vol. 197, 05.10.2018, blz. 1-7.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

TY - JOUR

T1 - Single cell trapping by capillary pumping using NOA81 replica moulded stencils

AU - Moonen, Emma

AU - Luttge, Regina

AU - Frimat, Jean Philippe

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AB - In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.

KW - Brain-on-a-chip

KW - NOA81

KW - Passive pumping

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