Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics

Tom M.J. Evers; Vahid Sheikhhassani; Mariëlle C. Haks; Cornelis Storm; Tom H.M. Ottenhoff; Alireza Mashaghi

doi:10.1016/j.isci.2021.103555

Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics

Tom M.J. Evers, Vahid Sheikhhassani, Mariëlle C. Haks, Cornelis Storm, Tom H.M. Ottenhoff, Alireza Mashaghi (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review

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Monocytes continuously adapt their shapes for proper circulation and elicitation of effective immune responses. Although these functions depend on the cell mechanical properties, the mechanical behavior of monocytes is still poorly understood and accurate physiologically relevant data on basic mechanical properties are lacking almost entirely. By combining several complementary single-cell force spectroscopy techniques, we report that the mechanical properties of human monocyte are strain-rate dependent, and that chemokines can induce alterations in viscoelastic behavior. In addition, our findings indicate that human monocytes are heterogeneous mechanically and this heterogeneity is regulated by chemokine CCL2. The technology presented here can be readily used to reveal mechanical complexity of the blood cell population in disease conditions, where viscoelastic properties may serve as physical biomarkers for disease progression and response to therapy.

Originele taal-2	Engels
Artikelnummer	103555
Aantal pagina's	13
Tijdschrift	iScience
Volume	25
Nummer van het tijdschrift	1
DOI's	https://doi.org/10.1016/j.isci.2021.103555
Status	Gepubliceerd - 21 jan. 2022

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Publisher Copyright:
© 2021 The Author(s)

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AM and TMJE acknowledge the support by the Netherlands Organization for Scientific Research (NWO-TTW, grant number 16249). We thank Barbara Scalvini (Leiden University) for assistance in data analysis and statistics. We also thank Mehrad Babaei (Leiden University), Anatoly Golovnev (Leiden University) and Valentin Romanov (Victor Chang Cardiac Research Institute) for helpful discussions. AM and TMJE acknowledge the support by the Netherlands Organization for Scientific Research (NWO-TTW, grant number 16249). We thank Barbara Scalvini (Leiden University) for assistance in data analysis and statistics. We also thank Mehrad Babaei (Leiden University), Anatoly Golovnev (Leiden University) and Valentin Romanov (Victor Chang Cardiac Research Institute) for helpful discussions. Conceptualization: AM; Methodology: TMJE, VS, MCH, CS, THMO, AM; Investigation: TMJE, VS, CS, AM; Visualization: TMJE, VS; Funding acquisition: AM; Project administration: AM; Supervision: AM; Writing ? original draft: TMJE, VS, AM; Writing ? review & editing: TMJE, VS, MCH, CS, THMO, AM. The authors declare no competing interests.

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10.1016/j.isci.2021.103555

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title = "Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics",

abstract = "Monocytes continuously adapt their shapes for proper circulation and elicitation of effective immune responses. Although these functions depend on the cell mechanical properties, the mechanical behavior of monocytes is still poorly understood and accurate physiologically relevant data on basic mechanical properties are lacking almost entirely. By combining several complementary single-cell force spectroscopy techniques, we report that the mechanical properties of human monocyte are strain-rate dependent, and that chemokines can induce alterations in viscoelastic behavior. In addition, our findings indicate that human monocytes are heterogeneous mechanically and this heterogeneity is regulated by chemokine CCL2. The technology presented here can be readily used to reveal mechanical complexity of the blood cell population in disease conditions, where viscoelastic properties may serve as physical biomarkers for disease progression and response to therapy.",

keywords = "Biomechanics, Cell biology, Immunology",

author = "Evers, {Tom M.J.} and Vahid Sheikhhassani and Haks, {Mari{\"e}lle C.} and Cornelis Storm and Ottenhoff, {Tom H.M.} and Alireza Mashaghi",

note = "Funding Information: AM and TMJE acknowledge the support by the Netherlands Organization for Scientific Research (NWO-TTW, grant number 16249). We thank Barbara Scalvini (Leiden University) for assistance in data analysis and statistics. We also thank Mehrad Babaei (Leiden University), Anatoly Golovnev (Leiden University) and Valentin Romanov (Victor Chang Cardiac Research Institute) for helpful discussions. Conceptualization: AM; Methodology: TMJE, VS, MCH, CS, THMO, AM; Investigation: TMJE, VS, CS, AM; Visualization: TMJE, VS; Funding acquisition: AM; Project administration: AM; Supervision: AM; Writing ? original draft: TMJE, VS, AM; Writing ? review & editing: TMJE, VS, MCH, CS, THMO, AM. The authors declare no competing interests. ",

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AU - Evers, Tom M.J.

AU - Sheikhhassani, Vahid

AU - Haks, Mariëlle C.

AU - Storm, Cornelis

AU - Ottenhoff, Tom H.M.

AU - Mashaghi, Alireza

N1 - Funding Information: AM and TMJE acknowledge the support by the Netherlands Organization for Scientific Research (NWO-TTW, grant number 16249). We thank Barbara Scalvini (Leiden University) for assistance in data analysis and statistics. We also thank Mehrad Babaei (Leiden University), Anatoly Golovnev (Leiden University) and Valentin Romanov (Victor Chang Cardiac Research Institute) for helpful discussions. Conceptualization: AM; Methodology: TMJE, VS, MCH, CS, THMO, AM; Investigation: TMJE, VS, CS, AM; Visualization: TMJE, VS; Funding acquisition: AM; Project administration: AM; Supervision: AM; Writing ? original draft: TMJE, VS, AM; Writing ? review & editing: TMJE, VS, MCH, CS, THMO, AM. The authors declare no competing interests.

PY - 2022/1/21

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N2 - Monocytes continuously adapt their shapes for proper circulation and elicitation of effective immune responses. Although these functions depend on the cell mechanical properties, the mechanical behavior of monocytes is still poorly understood and accurate physiologically relevant data on basic mechanical properties are lacking almost entirely. By combining several complementary single-cell force spectroscopy techniques, we report that the mechanical properties of human monocyte are strain-rate dependent, and that chemokines can induce alterations in viscoelastic behavior. In addition, our findings indicate that human monocytes are heterogeneous mechanically and this heterogeneity is regulated by chemokine CCL2. The technology presented here can be readily used to reveal mechanical complexity of the blood cell population in disease conditions, where viscoelastic properties may serve as physical biomarkers for disease progression and response to therapy.

AB - Monocytes continuously adapt their shapes for proper circulation and elicitation of effective immune responses. Although these functions depend on the cell mechanical properties, the mechanical behavior of monocytes is still poorly understood and accurate physiologically relevant data on basic mechanical properties are lacking almost entirely. By combining several complementary single-cell force spectroscopy techniques, we report that the mechanical properties of human monocyte are strain-rate dependent, and that chemokines can induce alterations in viscoelastic behavior. In addition, our findings indicate that human monocytes are heterogeneous mechanically and this heterogeneity is regulated by chemokine CCL2. The technology presented here can be readily used to reveal mechanical complexity of the blood cell population in disease conditions, where viscoelastic properties may serve as physical biomarkers for disease progression and response to therapy.

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Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics

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