Residue-specific incorporation of noncanonical amino acids for protein engineering

Mark B. van Eldijk, Jan C.M. van Hest

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukAcademicpeer review

1 Citaat (Scopus)

Samenvatting

The incorporation of noncanonical amino acids has given protein chemists access to an expanded repertoire of amino acids. This methodology has significantly broadened the scope of protein engineering allowing introduction of amino acids with non-native functionalities, such as bioorthogonal reactive handles (azides and alkynes) and hydrophobic fluorinated side chains. Here, we describe the efficient residue-specific replacement of methionine by azidonorleucine in an engineered green fluorescent protein using a bacterial expression system to introduce a single reactive site for the strain-promoted azide-alkyne cycloaddition.

Originele taal-2Engels
TitelNoncanonical amino acids
Subtitelmethods and protocols
RedacteurenE.A. Lemke
Plaats van productieNew York
UitgeverijHumana Press
Pagina's137-145
Aantal pagina's9
ISBN van elektronische versie 978-1-4939-7574-7
ISBN van geprinte versie978-1-4939-7573-0
DOI's
StatusGepubliceerd - 2018

Publicatie series

NaamMethods in Molecular Biology
Volume1728
ISSN van geprinte versie1064-3745

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  • Citeer dit

    van Eldijk, M. B., & van Hest, J. C. M. (2018). Residue-specific incorporation of noncanonical amino acids for protein engineering. In E. A. Lemke (editor), Noncanonical amino acids: methods and protocols (blz. 137-145). (Methods in Molecular Biology; Vol. 1728). Humana Press. https://doi.org/10.1007/978-1-4939-7574-7_8