TY - JOUR
T1 - RAFT Emulsion Polymerization as a Platform to Generate Well-Defined Biocompatible Latex Nanoparticles
AU - Gurnani, Pratik
AU - Sanchez-Cano, Carlos
AU - Abraham, Kristin
AU - Xandri-Monje, Helena
AU - Cook, Alexander B.
AU - Hartlieb, Matthias
AU - Lévi, Francis
AU - Dallmann, Robert
AU - Perrier, Sébastien
N1 - Funding Information:
The authors thank CRUK/EPSRC (C53561/A19933; P.G., C.S.-C., K.A., H.X.-M., F.L., S.P.), the German Research Foundation (DFG, GZ; HA 7725/1-1; M.H.), the Warwick Innovation Fund (R.D.), and the Royal Society Wolfson Merit Award (WM130055; S.P.) for financial support. The authors are grateful to LICOR Inc. for access to a Pearl Trilogy imaging system for in vivo experiments. The staff of the BSU at Warwick are thanked for animal husbandry.
Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/10
Y1 - 2018/10
N2 - Current approaches to generate core–shell nanoparticles for biomedical applications are limited by factors such as synthetic scalability and circulatory desorption of cytotoxic surfactants. Developments in controlled radical polymerization, particularly in dispersed states, represent a promising method of overcoming these challenges. In this work, well-defined PEGylated nanoparticles are synthesized using reversible addition fragmentation chain transfer emulsion polymerization to control particle size and surface composition and were further characterized with light scattering, electron microscopy, and size exclusion chromatography. Importantly, the nanoparticles are found to be tolerated both in vitro and in vivo, without the need for any purification after particle synthesis. Pharmacokinetic and biodistribution studies in mice, following intraperitoneal injection of the nanoparticles, reveal a long (>76 h) circulation time and accumulation in the liver.
AB - Current approaches to generate core–shell nanoparticles for biomedical applications are limited by factors such as synthetic scalability and circulatory desorption of cytotoxic surfactants. Developments in controlled radical polymerization, particularly in dispersed states, represent a promising method of overcoming these challenges. In this work, well-defined PEGylated nanoparticles are synthesized using reversible addition fragmentation chain transfer emulsion polymerization to control particle size and surface composition and were further characterized with light scattering, electron microscopy, and size exclusion chromatography. Importantly, the nanoparticles are found to be tolerated both in vitro and in vivo, without the need for any purification after particle synthesis. Pharmacokinetic and biodistribution studies in mice, following intraperitoneal injection of the nanoparticles, reveal a long (>76 h) circulation time and accumulation in the liver.
KW - biocompatible nanoparticles
KW - core–shell
KW - reversible addition fragmentation chain transfer emulsion polymerization
KW - tunable nanoparticles
KW - Caco-2 Cells
KW - Emulsions
KW - Humans
KW - Polymerization
KW - Male
KW - Materials Testing
KW - Latex/chemistry
KW - Animals
KW - Nanoparticles/chemistry
KW - Mice
UR - http://www.scopus.com/inward/record.url?scp=85052438662&partnerID=8YFLogxK
U2 - 10.1002/mabi.201800213
DO - 10.1002/mabi.201800213
M3 - Article
C2 - 30085410
AN - SCOPUS:85052438662
SN - 1616-5187
VL - 18
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 10
M1 - 1800213
ER -