TY - JOUR
T1 - Quantitative T2 mapping of the mouse heart by segmented MLEV phase-cycled T2 preparation
AU - Coolen, B.F.
AU - Simonis, F.F.J.
AU - Geelen, T.
AU - Moonen, R.P.M.
AU - Arslan, F. (Fatih)
AU - Paulis, L.E.M.
AU - Nicolay, K.
AU - Strijkers, G.J.
PY - 2014
Y1 - 2014
N2 - Purpose A high-quality, reproducible, multi-slice T2-mapping protocol for the mouse heart is presented. Methods A T2-prepared sequence with composite 90 and 180 radiofrequency pulses in a segmented MLEV phase cycling scheme was developed. The T2-mapping protocol was optimized using simulations and evaluated with phantoms. Results Repeatability for determination of myocardial T2 values was assessed in vivo in n=5 healthy mice on 2 different days. The average baseline T2 of the left ventricular myocardium was 22.5±1.7 ms. The repeatability coefficient for R2=1/T2 for measurements at different days was ¿R2=6.3 s-1. Subsequently, T2 mapping was applied in comparison to late-gadolinium-enhancement (LGE) imaging, to assess 1-day-old ischemia/reperfusion (IR) myocardial injury in n=8 mice. T2 in the infarcts was significantly higher than in remote tissue, whereas remote tissue was not significantly different from baseline. Infarct sizes based on T2 versus LGE showed strong correlation. To assess the time-course of T2 changes in the infarcts, T2 mapping was performed at day 1, 3, and 7 after IR injury in a separate group of mice (n=16). T2 was highest at day 3, in agreement with the expected time course of edema formation and resolution after myocardial infarction. Conclusion T2 prepared imaging provides high quality reproducible T2 maps of healthy and diseased mouse myocardium. Magn Reson Med 72:409-417, 2014. © 2013 Wiley Periodicals, Inc. Copyright © 2013 Wiley Periodicals, Inc.
AB - Purpose A high-quality, reproducible, multi-slice T2-mapping protocol for the mouse heart is presented. Methods A T2-prepared sequence with composite 90 and 180 radiofrequency pulses in a segmented MLEV phase cycling scheme was developed. The T2-mapping protocol was optimized using simulations and evaluated with phantoms. Results Repeatability for determination of myocardial T2 values was assessed in vivo in n=5 healthy mice on 2 different days. The average baseline T2 of the left ventricular myocardium was 22.5±1.7 ms. The repeatability coefficient for R2=1/T2 for measurements at different days was ¿R2=6.3 s-1. Subsequently, T2 mapping was applied in comparison to late-gadolinium-enhancement (LGE) imaging, to assess 1-day-old ischemia/reperfusion (IR) myocardial injury in n=8 mice. T2 in the infarcts was significantly higher than in remote tissue, whereas remote tissue was not significantly different from baseline. Infarct sizes based on T2 versus LGE showed strong correlation. To assess the time-course of T2 changes in the infarcts, T2 mapping was performed at day 1, 3, and 7 after IR injury in a separate group of mice (n=16). T2 was highest at day 3, in agreement with the expected time course of edema formation and resolution after myocardial infarction. Conclusion T2 prepared imaging provides high quality reproducible T2 maps of healthy and diseased mouse myocardium. Magn Reson Med 72:409-417, 2014. © 2013 Wiley Periodicals, Inc. Copyright © 2013 Wiley Periodicals, Inc.
U2 - 10.1002/mrm.24952
DO - 10.1002/mrm.24952
M3 - Article
C2 - 24186703
SN - 0740-3194
VL - 72
SP - 409
EP - 417
JO - Magnetic Resonance in Medicine
JF - Magnetic Resonance in Medicine
IS - 2
ER -