Quantification of skeletal muscle mitochondrial function by 31P magnetic resonance spectroscopy techniques: A quantitative review

G.J. Kemp, R.E. Ahmad, K. Nicolay, J.J. Prompers

    Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

    92 Citaten (Scopus)
    2 Downloads (Pure)

    Samenvatting

    Magnetic resonance spectroscopy (MRS) can give information about cellular metabolism in vivo which is difficult to obtain in other ways. In skeletal muscle, non-invasive 31P MRS measurements of the post-exercise recovery kinetics of pH, [PCr], [Pi] and [ADP] contain valuable information about muscle mitochondrial function and cellular pH homeostasis in vivo, but quantitative interpretation depends on understanding the underlying physiology. Here, by giving examples of the analysis of 31P MRS recovery data, by some simple computational simulation, and by extensively comparing data from published studies using both 31P MRS and invasive direct measurements of muscle O2 consumption in a common analytical framework, we consider what can be learnt quantitatively about mitochondrial metabolism in skeletal muscle using MRS-based methodology. We explore some technical and conceptual limitations of current methods, and point out some aspects of the physiology which are still incompletely understood.

    Originele taal-2Engels
    Pagina's (van-tot)107-144
    Aantal pagina's38
    TijdschriftActa Physiologica
    Volume213
    Nummer van het tijdschrift1
    DOI's
    StatusGepubliceerd - 1 jan 2015

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