Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging

Max L. Senders, Anu E. Meerwaldt, Mandy M.T. van Leent, Brenda L. Sanchez-Gaytan, Jan C. van de Voort, Yohana C. Toner, Alexander Maier, Emma D. Klein, Nathaniel A.T. Sullivan, Alexandros Marios Sofias, Hannah Groenen, Christopher Faries, Roderick S. Oosterwijk, Esther M. van Leeuwen, Francois Fay, Elena Chepurko, Thomas Reiner, Raphael Duivenvoorden, Lior Zangi, Rick M. DijkhuizenSjoerd Hak, Filip K. Swirski, Matthias Nahrendorf, Carlos Pérez-Medina, Abraham J.P. Teunissen, Zahi A. Fayad, Claudia Calcagno, Gustav J. Strijkers, Willem J.M. Mulder

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

1 Citaat (Scopus)

Samenvatting

Ischaemic heart disease evokes a complex immune response. However, tools to track the systemic behaviour and dynamics of leukocytes non-invasively in vivo are lacking. Here, we present a multimodal hot-spot imaging approach using an innovative high-density lipoprotein-derived nanotracer with a perfluoro-crown ether payload (19F-HDL) to allow myeloid cell tracking by 19F magnetic resonance imaging. The 19F-HDL nanotracer can additionally be labelled with zirconium-89 and fluorophores to detect myeloid cells by in vivo positron emission tomography imaging and optical modalities, respectively. Using our nanotracer in atherosclerotic mice with myocardial infarction, we observed rapid myeloid cell egress from the spleen and bone marrow by in vivo 19F-HDL magnetic resonance imaging. Concurrently, using ex vivo techniques, we showed that circulating pro-inflammatory myeloid cells accumulated in atherosclerotic plaques and at the myocardial infarct site. Our multimodality imaging approach is a valuable addition to the immunology toolbox, enabling the study of complex myeloid cell behaviour dynamically.

Originele taal-2Engels
Pagina's (van-tot)398-405
Aantal pagina's8
TijdschriftNature Nanotechnology
Volume15
Nummer van het tijdschrift5
DOI's
StatusGepubliceerd - 1 mei 2020

Vingerafdruk Duik in de onderzoeksthema's van 'Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging'. Samen vormen ze een unieke vingerafdruk.

  • Citeer dit

    Senders, M. L., Meerwaldt, A. E., van Leent, M. M. T., Sanchez-Gaytan, B. L., van de Voort, J. C., Toner, Y. C., Maier, A., Klein, E. D., Sullivan, N. A. T., Sofias, A. M., Groenen, H., Faries, C., Oosterwijk, R. S., van Leeuwen, E. M., Fay, F., Chepurko, E., Reiner, T., Duivenvoorden, R., Zangi, L., ... Mulder, W. J. M. (2020). Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging. Nature Nanotechnology, 15(5), 398-405. https://doi.org/10.1038/s41565-020-0642-4