TY - JOUR
T1 - Preparation and characterization of monolithic polymer columns for capillary electrochromatography
AU - Jiang, T.
AU - Jiskra, J.
AU - Claessens, H.A.
AU - Cramers, C.A.
PY - 2001/7/20
Y1 - 2001/7/20
N2 - A series of micro-monolithic columns with different porosities were prepared for capillary electrochromatography (CEC) by in-situ copolymerization of butyl methacrylate, ethylene glycol dimethacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in the presence of a porogen in fused-silica capillaries of 100 μm I.D. Different column porosities were obtained by changing the ratios of monomers to porogenic solvents. Columns were investigated and evaluated under both pressure-driven (high-performance liquid chromatography, HPLC) and electro-driven (capillary electrochromatography, CEC) conditions. Each column exhibited different efficiency and dependency on flow velocity under electro-driven conditions. Abnormally broad peaks for some relatively bulky molecules were observed. Possible explanations are discussed. The differences in column efficiency and retention behavior between the two eluent-driven modes were studied in detail. In addition, other column properties, such as morphology, porosity, stability and reproducibility, were extensively tested.
AB - A series of micro-monolithic columns with different porosities were prepared for capillary electrochromatography (CEC) by in-situ copolymerization of butyl methacrylate, ethylene glycol dimethacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in the presence of a porogen in fused-silica capillaries of 100 μm I.D. Different column porosities were obtained by changing the ratios of monomers to porogenic solvents. Columns were investigated and evaluated under both pressure-driven (high-performance liquid chromatography, HPLC) and electro-driven (capillary electrochromatography, CEC) conditions. Each column exhibited different efficiency and dependency on flow velocity under electro-driven conditions. Abnormally broad peaks for some relatively bulky molecules were observed. Possible explanations are discussed. The differences in column efficiency and retention behavior between the two eluent-driven modes were studied in detail. In addition, other column properties, such as morphology, porosity, stability and reproducibility, were extensively tested.
KW - Electrochromatography
KW - Micropore
KW - Monolithic polymer column
KW - Porosity
UR - http://www.scopus.com/inward/record.url?scp=0035919544&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)00982-7
DO - 10.1016/S0021-9673(01)00982-7
M3 - Article
C2 - 11510544
AN - SCOPUS:0035919544
SN - 0021-9673
VL - 923
SP - 215
EP - 227
JO - Journal of Chromatography, A
JF - Journal of Chromatography, A
IS - 1-2
ER -