Potential cultivation of lactobacillus pentosus from human breastmilk with rapid monitoring through the spectrophotometer method

Toan Nguyen-Sy, Guo Yong Yew, Kit Wayne Chew, Thi Dong Phuong Nguyen (Corresponding author), Thi Ngoc Thu Tran, Thi Dieu Huong Le, Chau Tuan Vo, Hoang Kim Pham Tran, Muhammad Mubashir (Corresponding author), Pau Loke Show (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

1 Citaat (Scopus)

Samenvatting

The present study focused on the development of a new method to determine the lag phase of Lactobacillus in breast milk which was attained during the 1st, 3rd, and 6th month (M1, M3, and M6). The colonies' phylogenetic analysis, derived from the 16S rRNA gene sequences, was evaluated with genus Lactobacillus pentosus and achieved a similarity value of 99%. Raman spectroscopy in optical densities of 600 nm (OD600) were used for six consecutive days to observe the changes of the cell growth rate. The values of OD600 were well fitted with the regression model. From this work, M1 was found to be the longest lag phase in 18 h, and it was 17% to 27% longer compared to M3 and M6, respectively. However, the samples of M3 and M6 showed the shortest duration in reaching 0.5 of OD600 nm (16 h) which was enhanced by 80% and 96% compared to M1, respectively. These studies will be of significance when applied in determining the bacteria growth curve and in assessing the growth behavior for the strain in human breast milk.

Originele taal-2Engels
Artikelnummer902
Aantal pagina's9
TijdschriftProcesses
Volume8
Nummer van het tijdschrift8
DOI's
StatusGepubliceerd - aug 2020

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