Plasmon rulers as a probe for real-time microsecond conformational dynamics of single molecules

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Biopolymers such as DNA, RNA, and proteins exploit conformational changes to modulate their function. Although state-of-the-art single-molecule approaches enable identification of conformational states, the transition path and metastable intermediates often remain elusive because they occur on microsecond time scales. Here we introduce a method to probe conformational dynamics with microsecond integration times based on a heterodimer of plasmonic particles. By combining Brownian dynamics and electromagnetic simulations, we find that integration times of 1 μs can be routinely achieved, providing the capability to identify short-lived intermediates and transition paths at the single-molecule level in real-time. Importantly, plasmon rulers require no specialized equipment but can be probed on existing fluorescence microscopes equipped with a fast camera. The approach combines the advantages of fluorescent probes (zero-force, parallelization) and mechanical probes such as optical tweezers (continuous microsecond integration times). They offer a unique opportunity to study conformational dynamics and compare measurements to full-atom simulations, where computational demands limit the simulation time.
TaalEngels
Pagina's7927-7934
Aantal pagina's8
TijdschriftNano Letters
Volume18
Nummer van het tijdschrift12
DOI's
StatusGepubliceerd - 12 dec 2018

Vingerafdruk

Molecules
probes
Optical tweezers
molecules
Biopolymers
RNA
Fluorescent Dyes
Microscopes
DNA
Fluorescence
Cameras
Proteins
Atoms
simulation
biopolymers
deoxyribonucleic acid
cameras
microscopes
electromagnetism
proteins

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    abstract = "Biopolymers such as DNA, RNA, and proteins exploit conformational changes to modulate their function. Although state-of-the-art single-molecule approaches enable identification of conformational states, the transition path and metastable intermediates often remain elusive because they occur on microsecond time scales. Here we introduce a method to probe conformational dynamics with microsecond integration times based on a heterodimer of plasmonic particles. By combining Brownian dynamics and electromagnetic simulations, we find that integration times of 1 μs can be routinely achieved, providing the capability to identify short-lived intermediates and transition paths at the single-molecule level in real-time. Importantly, plasmon rulers require no specialized equipment but can be probed on existing fluorescence microscopes equipped with a fast camera. The approach combines the advantages of fluorescent probes (zero-force, parallelization) and mechanical probes such as optical tweezers (continuous microsecond integration times). They offer a unique opportunity to study conformational dynamics and compare measurements to full-atom simulations, where computational demands limit the simulation time.",
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    Plasmon rulers as a probe for real-time microsecond conformational dynamics of single molecules. / Visser, Emiel W.A.; Horáček, Matěj; Zijlstra, Peter.

    In: Nano Letters, Vol. 18, Nr. 12, 12.12.2018, blz. 7927-7934.

    Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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