TY - GEN
T1 - Photoacoustic perfusion measurements
T2 - Photons Plus Ultrasound: Imaging and Sensing 2015
AU - Heres, H.M.
AU - Arabul, M.
AU - Tchang, B.C.Y.
AU - van de Vosse, F.N.
AU - Rutten, M.C.M.
AU - Lopata, R.G.P.
PY - 2015
Y1 - 2015
N2 - Ultrasound-based measurements using Doppler, contrast, and more recently photoacoustics (PA), have emerged as techniques for tissue perfusion measurements. In this study, the feasibility of in vitro perfusion measurements with a fully integrated, hand-held, photoacoustic probe was investigated and compared to Power Doppler (PD). Three cylindrical polyvinyl alcohol (PVA) phantoms were made (diameter = 15 mm) containing 100, 200 and 400 parallel polysulfone tubes (diameter = 0.2 mm), resulting in a perfused cross-sectional area of 1.8, 3.6 and 7.1% respectively. Each phantom was perfused with porcine blood (15 mL/min). Cross-sectional PA images (λ = 805nm, frame rate = 10Hz) and PD images (PRF = 750Hz) were acquired with a MyLab One and MyLab 70 scanner (Esaote, NL), respectively. Data were averaged over 70 frames. The average PA signal intensity was calculated in a region-of-interest of 4 mm by 6 mm. The percentage of colored PD pixels was measured in the entire phantom region. The average signal intensity of the PA images increased linearly with perfusion density, being 0.54 (± 0.01), 0.56 (± 0.01), 0.58 (± 0.01) with an average background signal of 0.53 in the three phantoms, respectively. For PD, the percentage of colored pixels in the phantom area (1.5% (± 0.2%), 4.4% (± 0.2%), 13.7% (± 0.8%)) also increased linearly. The preliminary results suggest that PA, like PD, is capable of detecting an increase of blood volume in tissue. In the future, in vivo measurements will be explored, although validation will be more complex.
AB - Ultrasound-based measurements using Doppler, contrast, and more recently photoacoustics (PA), have emerged as techniques for tissue perfusion measurements. In this study, the feasibility of in vitro perfusion measurements with a fully integrated, hand-held, photoacoustic probe was investigated and compared to Power Doppler (PD). Three cylindrical polyvinyl alcohol (PVA) phantoms were made (diameter = 15 mm) containing 100, 200 and 400 parallel polysulfone tubes (diameter = 0.2 mm), resulting in a perfused cross-sectional area of 1.8, 3.6 and 7.1% respectively. Each phantom was perfused with porcine blood (15 mL/min). Cross-sectional PA images (λ = 805nm, frame rate = 10Hz) and PD images (PRF = 750Hz) were acquired with a MyLab One and MyLab 70 scanner (Esaote, NL), respectively. Data were averaged over 70 frames. The average PA signal intensity was calculated in a region-of-interest of 4 mm by 6 mm. The percentage of colored PD pixels was measured in the entire phantom region. The average signal intensity of the PA images increased linearly with perfusion density, being 0.54 (± 0.01), 0.56 (± 0.01), 0.58 (± 0.01) with an average background signal of 0.53 in the three phantoms, respectively. For PD, the percentage of colored pixels in the phantom area (1.5% (± 0.2%), 4.4% (± 0.2%), 13.7% (± 0.8%)) also increased linearly. The preliminary results suggest that PA, like PD, is capable of detecting an increase of blood volume in tissue. In the future, in vivo measurements will be explored, although validation will be more complex.
KW - Perfusion
KW - Phantom
KW - Photoacoustic imaging
KW - Power Doppler
UR - http://www.scopus.com/inward/record.url?scp=84928578365&partnerID=8YFLogxK
U2 - 10.1117/12.2077556
DO - 10.1117/12.2077556
M3 - Conference contribution
AN - SCOPUS:84928578365
SN - 9781628414134
T3 - Proceedings of SPIE
BT - Progress in Biomedical Optics and Imaging
PB - SPIE
Y2 - 8 February 2015 through 10 February 2015
ER -