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Samenvatting
Under carbon source transitions, the intracellular pH of Saccharomyces cerevisiae is subject to change. Dynamics in pH modulate the activity of the glycolytic enzymes, resulting in a change in glycolytic flux and ultimately cell growth. To understand how pH affects the global behavior of glycolysis and ethanol fermentation, we measured the activity of the glycolytic and fermentative enzymes in S. cerevisiae under in vivo-like conditions at different pH. We demonstrate that glycolytic enzymes exhibit differential pH dependencies, and optima, in the pH range observed during carbon source transitions. The forward reaction of GAPDH shows the highest decrease in activity, 83%, during a simulated feast/famine regime upon glucose removal (cytosolic pH drop from 7.1 to 6.4). We complement our biochemical characterization of the glycolytic enzymes by fitting the V max to the progression curves of product formation or decay over time. The fitting analysis shows that the observed changes in enzyme activities require changes in V max , but changes in K m cannot be excluded. Our study highlights the relevance of pH as a key player in metabolic regulation and provides a large set of quantitative data that can be explored to improve our understanding of metabolism in dynamic environments.
Originele taal-2 | Engels |
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Pagina's (van-tot) | 6021-6037 |
Aantal pagina's | 17 |
Tijdschrift | FEBS Journal |
Volume | 289 |
Nummer van het tijdschrift | 19 |
Vroegere onlinedatum | 16 apr. 2022 |
DOI's | |
Status | Gepubliceerd - okt. 2022 |
Bibliografische nota
© 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.Financiering
The CEN.PK113‐5D strain was kindly provided by P. Kötter (Institut für Molekulare Biowissenschaften), Euroscarf, Frankfurt. We thank Chrats Melkonian, Frank Bruggeman, and Jurgen Haanstra for fruitful discussions and help with revision. We also acknowledge the financial support from the Netherlands Organization for Scientific Research (NWO), grant nr 737.016.001.
Financiers | Financiernummer |
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Nederlandse Organisatie voor Wetenschappelijk Onderzoek | 737.016.001 |
Vingerafdruk
Duik in de onderzoeksthema's van 'pH dependencies of glycolytic enzymes of yeast under in vivo-like assay conditions'. Samen vormen ze een unieke vingerafdruk.Projecten
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NWO Building Blocks of Life 737.016.001 Yeast 3M
van Riel, N. A. W. (Project Manager), Lao Martil, D. (Projectmedewerker), Teusink, B. (Project communicatie medewerker), Wahl, S. A. (Project communicatie medewerker) & Schmitz, J. P. J. (Project communicatie medewerker)
1/01/18 → 31/12/22
Project: Onderzoek direct