Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices

Mattia Ballerini; Mohammad Jouy Bar; Andrea Mainardi; Marco Rasponi; Giovanni Stefano Ugolini

doi:10.1007/978-1-0716-1693-2_2

Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices

Mattia Ballerini, Mohammad Jouy Bar, Andrea Mainardi, Marco Rasponi, Giovanni Stefano Ugolini

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Hoofdstuk › Academic › peer review

4 Citaten (Scopus)

Samenvatting

A relevant number of organ-on-chips is aimed at modeling epithelial/endothelial interfaces between tissue compartments. These barriers help tissue function either by protecting (e.g., endothelial blood–brain barrier) or by orchestrating relevant molecular exchanges (e.g., lung alveolar interface) in human organs. Models of these biological systems are aimed at characterizing the transport of molecules, drugs or drug carriers through these specific barriers. Multilayer microdevices are particularly appealing to this goal and techniques for embedding porous membranes within organ-on-chips are therefore at the basis of the development and use of such systems. Here, we discuss and provide procedures for embedding porous membranes within multilayer organ-on-chips. We present standard techniques involving both custom-made polydimethylsiloxane (PDMS) membranes and commercially available plastic membranes. In addition, we present a novel method for fabricating and bonding PDMS porous membranes by using a cost-effective epoxy resin in place of microfabricated silicon wafers as master molds.

Originele taal-2	Engels
Titel	Organ-on-a-Chip
Subtitel	Methods and Protocols
Redacteuren	Marco Rasponi
Plaats van productie	New York
Uitgeverij	Humana Press
Hoofdstuk	2
Pagina's	21-38
Aantal pagina's	18
ISBN van elektronische versie	978-1-0716-1693-2
ISBN van geprinte versie	978-1-0716-1692-5, 978-1-0716-1695-6
DOI's	https://doi.org/10.1007/978-1-0716-1693-2_2
Status	Gepubliceerd - 15 sep. 2021

Publicatie series

Naam	Methods in Molecular Biology (MIMB)
Uitgeverij	Springer
Volume	2373
ISSN van geprinte versie	1064-3745
ISSN van elektronische versie	1940-6029

Toegang tot document

10.1007/978-1-0716-1693-2_2

Andere bestanden en links

Link naar publicatie in Scopus

Best poster presentation prize
Jouy Bar, M. (Ontvanger), Ballerini, M. (Ontvanger), Nezi, L. (Ontvanger) & Rasponi, M. (Ontvanger), 10 sep. 2020
Prijs: Anders › Werk, activiteit of publicatie gerelateerde prijzen (lifetime, best paper, poster etc.) › Wetenschappelijk

4 Citaties
1 Poster

A Novel Soft Lithography Technique for Porous Membrane Fabrication as a Permeable Barrier in Gut- and Lung-on-Chip Devices
Jouy Bar, M., Ballerini, M., Nezi, L. & Rasponi, M., 19 sep. 2020.
Onderzoeksoutput: Bijdrage aan congres › Poster

Citeer dit

Ballerini, M., Jouy Bar, M., Mainardi, A., Rasponi, M., & Ugolini, G. S. (2021). Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices. In M. Rasponi (editor), Organ-on-a-Chip: Methods and Protocols (blz. 21-38). ( Methods in Molecular Biology (MIMB); Vol. 2373). Humana Press. https://doi.org/10.1007/978-1-0716-1693-2_2

@inbook{569215354381446597d939a0b4c486a5,

title = "Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices",

abstract = "A relevant number of organ-on-chips is aimed at modeling epithelial/endothelial interfaces between tissue compartments. These barriers help tissue function either by protecting (e.g., endothelial blood–brain barrier) or by orchestrating relevant molecular exchanges (e.g., lung alveolar interface) in human organs. Models of these biological systems are aimed at characterizing the transport of molecules, drugs or drug carriers through these specific barriers. Multilayer microdevices are particularly appealing to this goal and techniques for embedding porous membranes within organ-on-chips are therefore at the basis of the development and use of such systems. Here, we discuss and provide procedures for embedding porous membranes within multilayer organ-on-chips. We present standard techniques involving both custom-made polydimethylsiloxane (PDMS) membranes and commercially available plastic membranes. In addition, we present a novel method for fabricating and bonding PDMS porous membranes by using a cost-effective epoxy resin in place of microfabricated silicon wafers as master molds.",

keywords = "Microfluidics, Microdevices, Microfabrication techniques, microphysiological systems, Chemical/drug screening, Drug Development, Multilayer microdevices, Replica molding, Porous membranes, Organ-on-chip",

author = "Mattia Ballerini and \{Jouy Bar\}, Mohammad and Andrea Mainardi and Marco Rasponi and Ugolini, \{Giovanni Stefano\}",

year = "2021",

month = sep,

day = "15",

doi = "10.1007/978-1-0716-1693-2\_2",

language = "English",

isbn = "978-1-0716-1692-5",

series = " Methods in Molecular Biology (MIMB)",

publisher = "Humana Press",

pages = "21--38",

editor = "Marco Rasponi",

booktitle = "Organ-on-a-Chip",

address = "United States",

}

Ballerini, M, Jouy Bar, M, Mainardi, A, Rasponi, M & Ugolini, GS 2021, Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices. in M Rasponi (uitgave), Organ-on-a-Chip: Methods and Protocols. Methods in Molecular Biology (MIMB), vol. 2373, Humana Press, New York, blz. 21-38. https://doi.org/10.1007/978-1-0716-1693-2_2

Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices. / Ballerini, Mattia; Jouy Bar, Mohammad; Mainardi, Andrea et al.
Organ-on-a-Chip: Methods and Protocols. uitgave / Marco Rasponi. New York: Humana Press, 2021. blz. 21-38 ( Methods in Molecular Biology (MIMB); Vol. 2373).

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › Hoofdstuk › Academic › peer review

TY - CHAP

T1 - Organ-on-Chips for Studying Tissue Barriers

T2 - Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices

AU - Ballerini, Mattia

AU - Jouy Bar, Mohammad

AU - Mainardi, Andrea

AU - Rasponi, Marco

AU - Ugolini, Giovanni Stefano

PY - 2021/9/15

Y1 - 2021/9/15

N2 - A relevant number of organ-on-chips is aimed at modeling epithelial/endothelial interfaces between tissue compartments. These barriers help tissue function either by protecting (e.g., endothelial blood–brain barrier) or by orchestrating relevant molecular exchanges (e.g., lung alveolar interface) in human organs. Models of these biological systems are aimed at characterizing the transport of molecules, drugs or drug carriers through these specific barriers. Multilayer microdevices are particularly appealing to this goal and techniques for embedding porous membranes within organ-on-chips are therefore at the basis of the development and use of such systems. Here, we discuss and provide procedures for embedding porous membranes within multilayer organ-on-chips. We present standard techniques involving both custom-made polydimethylsiloxane (PDMS) membranes and commercially available plastic membranes. In addition, we present a novel method for fabricating and bonding PDMS porous membranes by using a cost-effective epoxy resin in place of microfabricated silicon wafers as master molds.

AB - A relevant number of organ-on-chips is aimed at modeling epithelial/endothelial interfaces between tissue compartments. These barriers help tissue function either by protecting (e.g., endothelial blood–brain barrier) or by orchestrating relevant molecular exchanges (e.g., lung alveolar interface) in human organs. Models of these biological systems are aimed at characterizing the transport of molecules, drugs or drug carriers through these specific barriers. Multilayer microdevices are particularly appealing to this goal and techniques for embedding porous membranes within organ-on-chips are therefore at the basis of the development and use of such systems. Here, we discuss and provide procedures for embedding porous membranes within multilayer organ-on-chips. We present standard techniques involving both custom-made polydimethylsiloxane (PDMS) membranes and commercially available plastic membranes. In addition, we present a novel method for fabricating and bonding PDMS porous membranes by using a cost-effective epoxy resin in place of microfabricated silicon wafers as master molds.

KW - Microfluidics

KW - Microdevices

KW - Microfabrication techniques

KW - microphysiological systems

KW - Chemical/drug screening

KW - Drug Development

KW - Multilayer microdevices

KW - Replica molding

KW - Porous membranes

KW - Organ-on-chip

UR - http://www.scopus.com/inward/record.url?scp=85115412489&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-1693-2_2

DO - 10.1007/978-1-0716-1693-2_2

M3 - Chapter

C2 - 34520004

SN - 978-1-0716-1692-5

SN - 978-1-0716-1695-6

T3 - Methods in Molecular Biology (MIMB)

SP - 21

EP - 38

BT - Organ-on-a-Chip

A2 - Rasponi, Marco

PB - Humana Press

CY - New York

ER -

Ballerini M, Jouy Bar M, Mainardi A, Rasponi M, Ugolini GS. Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices. In Rasponi M, redacteur, Organ-on-a-Chip: Methods and Protocols. New York: Humana Press. 2021. blz. 21-38. ( Methods in Molecular Biology (MIMB)). doi: 10.1007/978-1-0716-1693-2_2

Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices

Samenvatting

Publicatie series

Toegang tot document

Andere bestanden en links

Vingerafdruk

Prijzen

Best poster presentation prize

Onderzoekersoutput

A Novel Soft Lithography Technique for Porous Membrane Fabrication as a Permeable Barrier in Gut- and Lung-on-Chip Devices

Citeer dit