Optimizing charge state distribution is a prerequisite for accurate protein biomarker quantification with LC-MS/MS, as illustrated by hepcidin measurement

Ellen M.H. Schmitz, Niels M. Leijten, Joost L.J. van Dongen, Maarten A.C. Broeren, Lech G. Milroy, Luc Brunsveld, Volkher Scharnhorst, Daan van de Kerkhof

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1 Citaat (Scopus)

Uittreksel

Targeted quantification of protein biomarkers with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has great potential, but is still in its infancy. Therefore, we elucidated the influence of charge state distribution and matrix effects on accurate quantification, illustrated by the peptide hormone hepcidin. An LC-MS/MS assay for hepcidin, developed based on existing literature, was improved by using 5 mM ammonium formate buffer as mobile phase A and as an elution solution for solid phase extraction (SPE) to optimize the charge state distribution. After extensive analytical validation, focusing on interference and matrix effects, the clinical consequence of this method adjustment was studied by performing receiving operating characteristic (ROC)-curve analysis in patients with iron deficiency anemia (IDA, n=44), anemia of chronic disease (ACD, n=42) and non-anemic patients (n=93). By using a buffered solution during sample preparation and chromatography, the most abundant charge state was shifted from 4+ to 3+ and the charge state distribution was strongly stabilized. The matrix effects which occurred in the 4+ state were therefore avoided, eliminating bias in the low concentration range of hepcidin. Consequently, sensitivity, specificity and positive predictive value (PPV) for detection of IDA patients with the optimized assay (96%, 97%, 91%, respectively) were much better than for the original assay (73%, 70%, 44%, respectively). Fundamental improvements in LC-MS/MS assays greatly impact the accuracy of protein quantification. This is urgently required for improved diagnostic accuracy and clinical value, as illustrated by the validation of our hepcidin assay.

Originele taal-2Engels
Pagina's (van-tot)1490-1497
TijdschriftClinical Chemistry and Laboratory Medicine
Volume56
Nummer van het tijdschrift9
DOI's
StatusGepubliceerd - 19 mei 2018

Vingerafdruk

Hepcidins
Biomarkers
Assays
formic acid
Proteins
Iron-Deficiency Anemias
Peptide Hormones
Solid Phase Extraction
Tandem Mass Spectrometry
Liquid Chromatography
Liquid chromatography
Chromatography
Anemia
Buffers
Chronic Disease
Mass spectrometry
Sensitivity and Specificity
Iron

Citeer dit

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title = "Optimizing charge state distribution is a prerequisite for accurate protein biomarker quantification with LC-MS/MS, as illustrated by hepcidin measurement",
abstract = "Targeted quantification of protein biomarkers with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has great potential, but is still in its infancy. Therefore, we elucidated the influence of charge state distribution and matrix effects on accurate quantification, illustrated by the peptide hormone hepcidin. An LC-MS/MS assay for hepcidin, developed based on existing literature, was improved by using 5 mM ammonium formate buffer as mobile phase A and as an elution solution for solid phase extraction (SPE) to optimize the charge state distribution. After extensive analytical validation, focusing on interference and matrix effects, the clinical consequence of this method adjustment was studied by performing receiving operating characteristic (ROC)-curve analysis in patients with iron deficiency anemia (IDA, n=44), anemia of chronic disease (ACD, n=42) and non-anemic patients (n=93). By using a buffered solution during sample preparation and chromatography, the most abundant charge state was shifted from 4+ to 3+ and the charge state distribution was strongly stabilized. The matrix effects which occurred in the 4+ state were therefore avoided, eliminating bias in the low concentration range of hepcidin. Consequently, sensitivity, specificity and positive predictive value (PPV) for detection of IDA patients with the optimized assay (96{\%}, 97{\%}, 91{\%}, respectively) were much better than for the original assay (73{\%}, 70{\%}, 44{\%}, respectively). Fundamental improvements in LC-MS/MS assays greatly impact the accuracy of protein quantification. This is urgently required for improved diagnostic accuracy and clinical value, as illustrated by the validation of our hepcidin assay.",
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author = "Schmitz, {Ellen M.H.} and Leijten, {Niels M.} and {van Dongen}, {Joost L.J.} and Broeren, {Maarten A.C.} and Milroy, {Lech G.} and Luc Brunsveld and Volkher Scharnhorst and {van de Kerkhof}, Daan",
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Optimizing charge state distribution is a prerequisite for accurate protein biomarker quantification with LC-MS/MS, as illustrated by hepcidin measurement. / Schmitz, Ellen M.H.; Leijten, Niels M.; van Dongen, Joost L.J.; Broeren, Maarten A.C.; Milroy, Lech G.; Brunsveld, Luc; Scharnhorst, Volkher; van de Kerkhof, Daan.

In: Clinical Chemistry and Laboratory Medicine, Vol. 56, Nr. 9, 19.05.2018, blz. 1490-1497.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

TY - JOUR

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AU - Schmitz, Ellen M.H.

AU - Leijten, Niels M.

AU - van Dongen, Joost L.J.

AU - Broeren, Maarten A.C.

AU - Milroy, Lech G.

AU - Brunsveld, Luc

AU - Scharnhorst, Volkher

AU - van de Kerkhof, Daan

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