Oligonucleotide-protamine-albumin nanoparticles: Protamine sulfate causes drastic size reduction

G. Mayer; V. Vogel; J. Weyermann; D. Lochmann; J.A. Broek, van den; C. Tziatzios; W. Haase; D. Wouters; U.S. Schubert; A. Zimmer; J. Kreuter; D. Schubert

doi:10.1016/j.jconrel.2005.04.019

Oligonucleotide-protamine-albumin nanoparticles: Protamine sulfate causes drastic size reduction

G. Mayer, V. Vogel, J. Weyermann, D. Lochmann, J.A. Broek, van den, C. Tziatzios, W. Haase, D. Wouters, U.S. Schubert, A. Zimmer, J. Kreuter, D. Schubert

Chemical Engineering and Chemistry

Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review

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Nanoparticles prepared by self-assembly from oligonucleotides (ONs), protamine free base, and human serum albumin (bternary proticlesQ) are spheres of diameters around 200 nm. Substitution of the protamine free base by protamine sulfate leads to proticles of only around 40 nm in diameter with otherwise unchanged properties. The availability of drug delivery systems of very similar composition but grossly different size may be advantageous when dealing with cells which show size-dependent particle uptake. These nanoparticles are promising candidates for ON delivery to cells because of the following reasons: (1) They are stable for several hours in solutions of up to physiological ionic strength; (2) they are efficiently taken up by cells; (3) after cellular uptake, they easily release the ONs even when these are present as phosphorothioates.

Originele taal-2	Engels
Pagina's (van-tot)	181-187
Aantal pagina's	12
Tijdschrift	Journal of Controlled Release
Volume	106
Nummer van het tijdschrift	1-2
DOI's	https://doi.org/10.1016/j.jconrel.2005.04.019
Status	Gepubliceerd - 2005

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Mayer, G., Vogel, V., Weyermann, J., Lochmann, D., Broek, van den, J. A., Tziatzios, C., Haase, W., Wouters, D., Schubert, U. S., Zimmer, A., Kreuter, J., & Schubert, D. (2005). Oligonucleotide-protamine-albumin nanoparticles: Protamine sulfate causes drastic size reduction. Journal of Controlled Release, 106(1-2), 181-187. https://doi.org/10.1016/j.jconrel.2005.04.019

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title = "Oligonucleotide-protamine-albumin nanoparticles: Protamine sulfate causes drastic size reduction",

abstract = "Nanoparticles prepared by self-assembly from oligonucleotides (ONs), protamine free base, and human serum albumin (bternary proticlesQ) are spheres of diameters around 200 nm. Substitution of the protamine free base by protamine sulfate leads to proticles of only around 40 nm in diameter with otherwise unchanged properties. The availability of drug delivery systems of very similar composition but grossly different size may be advantageous when dealing with cells which show size-dependent particle uptake. These nanoparticles are promising candidates for ON delivery to cells because of the following reasons: (1) They are stable for several hours in solutions of up to physiological ionic strength; (2) they are efficiently taken up by cells; (3) after cellular uptake, they easily release the ONs even when these are present as phosphorothioates.",

author = "G. Mayer and V. Vogel and J. Weyermann and D. Lochmann and {Broek, van den}, J.A. and C. Tziatzios and W. Haase and D. Wouters and U.S. Schubert and A. Zimmer and J. Kreuter and D. Schubert",

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T1 - Oligonucleotide-protamine-albumin nanoparticles: Protamine sulfate causes drastic size reduction

AU - Mayer, G.

AU - Vogel, V.

AU - Weyermann, J.

AU - Lochmann, D.

AU - Broek, van den, J.A.

AU - Tziatzios, C.

AU - Haase, W.

AU - Wouters, D.

AU - Schubert, U.S.

AU - Zimmer, A.

AU - Kreuter, J.

AU - Schubert, D.

PY - 2005

Y1 - 2005

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AB - Nanoparticles prepared by self-assembly from oligonucleotides (ONs), protamine free base, and human serum albumin (bternary proticlesQ) are spheres of diameters around 200 nm. Substitution of the protamine free base by protamine sulfate leads to proticles of only around 40 nm in diameter with otherwise unchanged properties. The availability of drug delivery systems of very similar composition but grossly different size may be advantageous when dealing with cells which show size-dependent particle uptake. These nanoparticles are promising candidates for ON delivery to cells because of the following reasons: (1) They are stable for several hours in solutions of up to physiological ionic strength; (2) they are efficiently taken up by cells; (3) after cellular uptake, they easily release the ONs even when these are present as phosphorothioates.

U2 - 10.1016/j.jconrel.2005.04.019

DO - 10.1016/j.jconrel.2005.04.019

M3 - Article

C2 - 16002173

SN - 0168-3659

VL - 106

SP - 181

EP - 187

JO - Journal of Controlled Release

JF - Journal of Controlled Release

IS - 1-2

ER -

Oligonucleotide-protamine-albumin nanoparticles: Protamine sulfate causes drastic size reduction

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