The Eindhoven scanning proton microprobe enables the determination of the ion content of heart tissue on a sub-cellular scale. It is shown that intra-cellular elemental concentrations can be determined. Measurements are carried out for physiological and patho-physiological rat heart muscle tissue. Important alterations in Na and K concentrations are reported as measured with PIXE, RBS and NFS techniques employing a variable energy cyclotron (3–30 MeV). However, quantitative intra-cellular determinations are hampered by the presence of extra-cellular space (ECS). For this purpose, cobaltic ethylene-diamine-tetra-acetic-acid (Co(III)EDTA) was used as an exogenic ECS marker. The intra-cellular ion concentrations of normoxic tissue after correction for the ECS agree well with the literature values.
|Tijdschrift||Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms|
|Nummer van het tijdschrift||1-4|
|Status||Gepubliceerd - 1999|