Nanogroove-enhanced hydrogel scaffolds for 3D neuronal cell culture: an easy access brain-on-chip model

Alex Bastiaens, Sijia Xie, Regina Luttge (Corresponding author)

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In order to better understand the brain and brain diseases, in vitro human brain models need to include not only a chemically and physically relevant microenvironment, but also structural network complexity. This complexity reflects the hierarchical architecture in brain tissue. Here, a method has been developed that adds complexity to a 3D cell culture by means of nanogrooved substrates. SH-SY5Y cells were grown on these nanogrooved substrates and covered with Matrigel, a hydrogel. To quantitatively analyze network behavior in 2D neuronal cell cultures, we previously developed an automated image-based screening method. We first investigated if this method was applicable to 3D primary rat brain cortical (CTX) cell cultures. Since the method was successfully applied to these pilot data, a proof of principle in a reductionist human brain cell model was attempted, using the SH-SY5Y cell line. The results showed that these cells also create an aligned network in the 3D microenvironment by maintaining a certain degree of guidance by the nanogrooved topography in the z-direction. These results indicate that nanogrooves enhance the structural complexity of 3D neuronal cell cultures for both CTX and human SH-SY5Y cultures, providing a basis for further development of an easy access brain-on-chip model.

Originele taal-2Engels
Artikelnummer638
Aantal pagina's17
TijdschriftMicromachines
Volume10
Nummer van het tijdschrift10
DOI's
StatusGepubliceerd - 1 okt 2019

Vingerafdruk

Scaffolds (biology)
Cell culture
Hydrogels
Brain
Brain models
Substrates
Topography
Rats
Screening
Cells
Tissue

Citeer dit

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title = "Nanogroove-enhanced hydrogel scaffolds for 3D neuronal cell culture: an easy access brain-on-chip model",
abstract = "In order to better understand the brain and brain diseases, in vitro human brain models need to include not only a chemically and physically relevant microenvironment, but also structural network complexity. This complexity reflects the hierarchical architecture in brain tissue. Here, a method has been developed that adds complexity to a 3D cell culture by means of nanogrooved substrates. SH-SY5Y cells were grown on these nanogrooved substrates and covered with Matrigel, a hydrogel. To quantitatively analyze network behavior in 2D neuronal cell cultures, we previously developed an automated image-based screening method. We first investigated if this method was applicable to 3D primary rat brain cortical (CTX) cell cultures. Since the method was successfully applied to these pilot data, a proof of principle in a reductionist human brain cell model was attempted, using the SH-SY5Y cell line. The results showed that these cells also create an aligned network in the 3D microenvironment by maintaining a certain degree of guidance by the nanogrooved topography in the z-direction. These results indicate that nanogrooves enhance the structural complexity of 3D neuronal cell cultures for both CTX and human SH-SY5Y cultures, providing a basis for further development of an easy access brain-on-chip model.",
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Nanogroove-enhanced hydrogel scaffolds for 3D neuronal cell culture : an easy access brain-on-chip model. / Bastiaens, Alex; Xie, Sijia; Luttge, Regina (Corresponding author).

In: Micromachines, Vol. 10, Nr. 10, 638, 01.10.2019.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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