Monitoring the behaviour of 4-ketocyclophosphamide versus cyclophosphamide during capillary gas chromatography by mass spectrometry

E.A. Bruijn, de, A.T. Oosterom, van, P.A. Leclercq, J.W. Haan, de, L.J.M. Ven, van de, U.R. Tjaden

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Samenvatting

Capillary Gas Chromatography (CGC) is capable of determining underivatized cyclophosphamide (CPA) using SCOT OV 275 columns. Then CPA is subjected to in situ degradation resulting in formation of a cyclization product which can be determined selectively in biological fluids. In routine bioanalysis however cyclization products of CPA metabolites might interfere, e.g. 4-keto CPA. In the present study possible formation of cyclization products of 4-keto CPA similar to CPA was monitored by Mass Spectrometry. Cyclization of 4-keto CPA in situ was demonstrated to occur, resulting in a product similar to that of CPA. Both cyclization products could be determined selectively and it appeared that in situ cyclization of 4-keto CPA was negligible (
Originele taal-2Engels
Pagina's (van-tot)643-647
TijdschriftBiomedical & Environmental Mass Spectrometry
Volume14
Nummer van het tijdschrift11
DOI's
StatusGepubliceerd - 1987

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Bruijn, de, E. A., Oosterom, van, A. T., Leclercq, P. A., Haan, de, J. W., Ven, van de, L. J. M., & Tjaden, U. R. (1987). Monitoring the behaviour of 4-ketocyclophosphamide versus cyclophosphamide during capillary gas chromatography by mass spectrometry. Biomedical & Environmental Mass Spectrometry, 14(11), 643-647. https://doi.org/10.1002/bms.1200141113