Microfluidics for cell-based high throughput screening platforms : a review

Guansheng Du, Q. Fang, Jaap den Toonder

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

131 Citaten (Scopus)
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Samenvatting

In the last decades, the basic techniques of microfluidics for the study of cells such as cell culture, cell separation, and cell lysis, have been well developed. Based on cell handling techniques, microfluidics has been widely applied in the field of PCR (Polymerase Chain Reaction), immunoassays, organ-on-chip, stem cell research, and analysis and identification of circulating tumor cells. As a major step in drug discovery, high-throughput screening allows rapid analysis of thousands of chemical, biochemical, genetic or pharmacological tests in parallel. In this review, we summarize the application of microfluidics in cell based high throughput screening. The screening methods mentioned in this paper include approaches using the perfusion flow mode, the droplet mode, and the microarray mode. We also discuss the future development of microfluidic based high throughput screening platform for drug discovery.
Originele taal-2Engels
Pagina's (van-tot)36-50
TijdschriftAnalytica Chimica Acta
Volume903
DOI's
StatusGepubliceerd - 22 nov 2015

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