Micro-scaled topographies direct differentiation of human epidermal stem cells

Sebastiaan Zijl, Aliaksei S. Vasilevich, Priyalakshmi Viswanathan, Ayelen Luna Helling, Nick R.M. Beijer, Gernot Walko, Ciro Chiappini, Jan de Boer, Fiona M. Watt (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

2 Citaties (Scopus)

Uittreksel

Human epidermal stem cells initiate terminal differentiation when spreading is restricted on ECM-coated micropatterned islands, soft hydrogels or hydrogel-nanoparticle composites with high nanoparticle spacing. The effect of substrate topography, however, is incompletely understood. To explore this, primary human keratinocytes enriched for stem cells were seeded on a topographical library with over 2000 different topographies in the micrometre range. Twenty-four hours later the proportion of cells expressing the differentiation marker transglutaminase-1 was determined by high content imaging. As predicted, topographies that prevented spreading promoted differentiation. However, we also identified topographies that supported differentiation of highly spread cells. Topographies supporting differentiation of spread cells were more irregular than those supporting differentiation of round cells. Low topography coverage promoted differentiation of spread cells, whereas high coverage promoted differentiation of round cells. Based on these observations we fabricated a topography in 6-well plate format that supported differentiation of spread cells, enabling us to examine cell responses at higher resolution. We found that differentiated spread cells did not assemble significant numbers of hemidesmosomes, focal adhesions, adherens junctions, desmosomes or tight junctions. They did, however, organise the actin cytoskeleton in response to the topographies. Rho kinase inhibition and blebbistatin treatment blocked the differentiation of spread cells, whereas SRF inhibition did not. These observations suggest a potential role for actin polymerization and actomyosin contraction in the topography-induced differentiation of spread cells.
TaalEngels
Pagina's133-145
Aantal pagina's13
TijdschriftActa Biomaterialia
Volume84
DOI's
StatusGepubliceerd - 15 jan 2019

Trefwoorden

    Citeer dit

    Zijl, S., Vasilevich, A. S., Viswanathan, P., Helling, A. L., Beijer, N. R. M., Walko, G., ... Watt, F. M. (2019). Micro-scaled topographies direct differentiation of human epidermal stem cells. Acta Biomaterialia, 84, 133-145. DOI: 10.1016/j.actbio.2018.12.003
    Zijl, Sebastiaan ; Vasilevich, Aliaksei S. ; Viswanathan, Priyalakshmi ; Helling, Ayelen Luna ; Beijer, Nick R.M. ; Walko, Gernot ; Chiappini, Ciro ; de Boer, Jan ; Watt, Fiona M./ Micro-scaled topographies direct differentiation of human epidermal stem cells. In: Acta Biomaterialia. 2019 ; Vol. 84. blz. 133-145
    @article{99f6014c737b4d9daffcfe70e045a73f,
    title = "Micro-scaled topographies direct differentiation of human epidermal stem cells",
    abstract = "Human epidermal stem cells initiate terminal differentiation when spreading is restricted on ECM-coated micropatterned islands, soft hydrogels or hydrogel-nanoparticle composites with high nanoparticle spacing. The effect of substrate topography, however, is incompletely understood. To explore this, primary human keratinocytes enriched for stem cells were seeded on a topographical library with over 2000 different topographies in the micrometre range. Twenty-four hours later the proportion of cells expressing the differentiation marker transglutaminase-1 was determined by high content imaging. As predicted, topographies that prevented spreading promoted differentiation. However, we also identified topographies that supported differentiation of highly spread cells. Topographies supporting differentiation of spread cells were more irregular than those supporting differentiation of round cells. Low topography coverage promoted differentiation of spread cells, whereas high coverage promoted differentiation of round cells. Based on these observations we fabricated a topography in 6-well plate format that supported differentiation of spread cells, enabling us to examine cell responses at higher resolution. We found that differentiated spread cells did not assemble significant numbers of hemidesmosomes, focal adhesions, adherens junctions, desmosomes or tight junctions. They did, however, organise the actin cytoskeleton in response to the topographies. Rho kinase inhibition and blebbistatin treatment blocked the differentiation of spread cells, whereas SRF inhibition did not. These observations suggest a potential role for actin polymerization and actomyosin contraction in the topography-induced differentiation of spread cells.",
    keywords = "Topography, Keratinocytes, Epidermal stem cells, Actin polymerization, Differentiation, Actomyosin contractility",
    author = "Sebastiaan Zijl and Vasilevich, {Aliaksei S.} and Priyalakshmi Viswanathan and Helling, {Ayelen Luna} and Beijer, {Nick R.M.} and Gernot Walko and Ciro Chiappini and {de Boer}, Jan and Watt, {Fiona M.}",
    year = "2019",
    month = "1",
    day = "15",
    doi = "10.1016/j.actbio.2018.12.003",
    language = "English",
    volume = "84",
    pages = "133--145",
    journal = "Acta Biomaterialia",
    issn = "1742-7061",
    publisher = "Elsevier",

    }

    Zijl, S, Vasilevich, AS, Viswanathan, P, Helling, AL, Beijer, NRM, Walko, G, Chiappini, C, de Boer, J & Watt, FM 2019, 'Micro-scaled topographies direct differentiation of human epidermal stem cells' Acta Biomaterialia, vol. 84, blz. 133-145. DOI: 10.1016/j.actbio.2018.12.003

    Micro-scaled topographies direct differentiation of human epidermal stem cells. / Zijl, Sebastiaan; Vasilevich, Aliaksei S.; Viswanathan, Priyalakshmi; Helling, Ayelen Luna; Beijer, Nick R.M.; Walko, Gernot; Chiappini, Ciro; de Boer, Jan; Watt, Fiona M. (Corresponding author).

    In: Acta Biomaterialia, Vol. 84, 15.01.2019, blz. 133-145.

    Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

    TY - JOUR

    T1 - Micro-scaled topographies direct differentiation of human epidermal stem cells

    AU - Zijl,Sebastiaan

    AU - Vasilevich,Aliaksei S.

    AU - Viswanathan,Priyalakshmi

    AU - Helling,Ayelen Luna

    AU - Beijer,Nick R.M.

    AU - Walko,Gernot

    AU - Chiappini,Ciro

    AU - de Boer,Jan

    AU - Watt,Fiona M.

    PY - 2019/1/15

    Y1 - 2019/1/15

    N2 - Human epidermal stem cells initiate terminal differentiation when spreading is restricted on ECM-coated micropatterned islands, soft hydrogels or hydrogel-nanoparticle composites with high nanoparticle spacing. The effect of substrate topography, however, is incompletely understood. To explore this, primary human keratinocytes enriched for stem cells were seeded on a topographical library with over 2000 different topographies in the micrometre range. Twenty-four hours later the proportion of cells expressing the differentiation marker transglutaminase-1 was determined by high content imaging. As predicted, topographies that prevented spreading promoted differentiation. However, we also identified topographies that supported differentiation of highly spread cells. Topographies supporting differentiation of spread cells were more irregular than those supporting differentiation of round cells. Low topography coverage promoted differentiation of spread cells, whereas high coverage promoted differentiation of round cells. Based on these observations we fabricated a topography in 6-well plate format that supported differentiation of spread cells, enabling us to examine cell responses at higher resolution. We found that differentiated spread cells did not assemble significant numbers of hemidesmosomes, focal adhesions, adherens junctions, desmosomes or tight junctions. They did, however, organise the actin cytoskeleton in response to the topographies. Rho kinase inhibition and blebbistatin treatment blocked the differentiation of spread cells, whereas SRF inhibition did not. These observations suggest a potential role for actin polymerization and actomyosin contraction in the topography-induced differentiation of spread cells.

    AB - Human epidermal stem cells initiate terminal differentiation when spreading is restricted on ECM-coated micropatterned islands, soft hydrogels or hydrogel-nanoparticle composites with high nanoparticle spacing. The effect of substrate topography, however, is incompletely understood. To explore this, primary human keratinocytes enriched for stem cells were seeded on a topographical library with over 2000 different topographies in the micrometre range. Twenty-four hours later the proportion of cells expressing the differentiation marker transglutaminase-1 was determined by high content imaging. As predicted, topographies that prevented spreading promoted differentiation. However, we also identified topographies that supported differentiation of highly spread cells. Topographies supporting differentiation of spread cells were more irregular than those supporting differentiation of round cells. Low topography coverage promoted differentiation of spread cells, whereas high coverage promoted differentiation of round cells. Based on these observations we fabricated a topography in 6-well plate format that supported differentiation of spread cells, enabling us to examine cell responses at higher resolution. We found that differentiated spread cells did not assemble significant numbers of hemidesmosomes, focal adhesions, adherens junctions, desmosomes or tight junctions. They did, however, organise the actin cytoskeleton in response to the topographies. Rho kinase inhibition and blebbistatin treatment blocked the differentiation of spread cells, whereas SRF inhibition did not. These observations suggest a potential role for actin polymerization and actomyosin contraction in the topography-induced differentiation of spread cells.

    KW - Topography

    KW - Keratinocytes

    KW - Epidermal stem cells

    KW - Actin polymerization

    KW - Differentiation

    KW - Actomyosin contractility

    U2 - 10.1016/j.actbio.2018.12.003

    DO - 10.1016/j.actbio.2018.12.003

    M3 - Article

    VL - 84

    SP - 133

    EP - 145

    JO - Acta Biomaterialia

    T2 - Acta Biomaterialia

    JF - Acta Biomaterialia

    SN - 1742-7061

    ER -

    Zijl S, Vasilevich AS, Viswanathan P, Helling AL, Beijer NRM, Walko G et al. Micro-scaled topographies direct differentiation of human epidermal stem cells. Acta Biomaterialia. 2019 jan 15;84:133-145. Beschikbaar vanaf, DOI: 10.1016/j.actbio.2018.12.003