A proton microprobe in combination with Proton Induced X-ray Emission (micro-PIXE) and Fourier transform–infrared (FT–IR) microscopy are used for examination of the elemental composition and structure of inorganic deposits formed in cell cultures of rat bone marrow. The results show that micro-PIXE is well suited for prior recognition of the localization and for the determination of the elemental composition of the deposits while FT–IR microscopy may be used to determine the structure of deposits in situ with a spatial resolution of about 20 µm. It is demonstrated that nodules in the cell culture are composed of different Ca compounds. In contrast to physiological mineralization, Ca–P compounds do not dominate the mineral phase. Discrepancies are observed between histomorphometric and micro-PIXE data.
|Tijdschrift||Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms|
|Nummer van het tijdschrift||1-4|
|Status||Gepubliceerd - 1998|