Metal ion-induced self-assembly and packaging of CCMV nanocapsules

Lise Schoonen, Mark B. van Eldijk, Jan C.M. van Hest

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukAcademicpeer review

1 Citaat (Scopus)

Samenvatting

The controlled self-assembly of protein cages is vital for the use of these nanocompartments in biomedical and nanotechnological applications. Recently, we showed that by combining different structural peptide elements, it is possible to assemble viral capsid proteins in distinct well-defined morphologies. In this chapter, a triblock copolypeptide is discussed, consisting of a metal ion-coordinating hexahistidine tag, a stimulus-responsive elastin-like polypeptide and a pH-responsive self-assembling viral capsid protein. This protein is able to form two different types of capsids, depending on the assembly pathway that is followed. Here, we focus on the metal ion-induced assembly process and describe the relevant experimental procedures to induce and utilize this assembly behavior.

Originele taal-2Engels
TitelProtein scaffolds
Subtiteldesign, synthesis, and applications
RedacteurenA.K. Udit
Plaats van productieDordrecht
UitgeverijSpringer
Pagina's57-67
Aantal pagina's11
ISBN van elektronische versie978-1-4939-7893-9
ISBN van geprinte versie978-1-4939-7892-2
DOI's
StatusGepubliceerd - 5 jun 2018

Publicatie series

NaamMethods in Molecular Biology
Volume1798
ISSN van geprinte versie1064-3745

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  • Citeer dit

    Schoonen, L., van Eldijk, M. B., & van Hest, J. C. M. (2018). Metal ion-induced self-assembly and packaging of CCMV nanocapsules. In A. K. Udit (editor), Protein scaffolds: design, synthesis, and applications (blz. 57-67). (Methods in Molecular Biology; Vol. 1798). Springer. https://doi.org/10.1007/978-1-4939-7893-9_5