Interaction between electrical stimulation, protein coating and matrix elasticity: a complex effect on muscle fibre maturation

K.J.M. Boonen, D.W.J. Schaft, van der, F.P.T. Baaijens, M.J. Post

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

16 Citaten (Scopus)

Samenvatting

In skeletal muscle tissue engineering, it remains a challenge to produce mature, functional muscle tissue. Mimicking the in vivo niche in in vitro culture might overcome this problem. Niche components include, for example, extracellular matrix proteins, neighbouring cells, growth factors and physical factors such as the elasticity of the matrix. Previously, we showed the effects of matrix stiffness and protein coating on proliferation and differentiation of muscle progenitor cells in a two-dimensional (2D) situation. In the present study we have investigated the additional effect of electrical stimulation. More precisely, we investigated the effect of electrical stimulation on primary myoblast maturation when cultured on top of Matrigel™- or laminin-coated substrates with varying elasticities. The effect of electrical stimulation on differentiation and maturation was found to be dependent on coating and stiffness. Although electrical stimulation enhanced myoblast maturation, the effect was mild. We therefore conclude that, with the current regimen, electrical stimulation is not essential to create functional, mature muscle tissue.
Originele taal-2Engels
Pagina's (van-tot)60-68
TijdschriftJournal of Tissue Engineering and Regenerative Medicine
Volume5
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 2011

Vingerafdruk Duik in de onderzoeksthema's van 'Interaction between electrical stimulation, protein coating and matrix elasticity: a complex effect on muscle fibre maturation'. Samen vormen ze een unieke vingerafdruk.

Citeer dit