In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates

E. Lamers, X.F. Walboomers, M. Domanski, L. Prodanov, J. Melis, R. Luttge, A.J.A. Winnubst, J.M. Anderson, J.G.E. Gardeniers, J.A. Jansen

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

31 Citaties (Scopus)
1 Downloads (Pure)

Uittreksel

The immune response to an implanted biomaterial is orchestrated by macrophages. In this study various nanogrooved patterns were created by using laser interference lithography and reactive ion etching. The created nanogrooves mimic the natural extracellular matrix environment. Macrophage cell culture demonstrated that interleukin 1ß and TNF-a cytokine production were upregulated on nanogrooved substrates. In vivo subcutaneous implantation in a validated mouse cage model for 14 days demonstrated that nanogrooves enhanced and guided cell adhesion, and few multinucleated cells were formed. In agreement with the in vitro results, cytokine production was found to be nanogroove dependent, as interleukin 1ß, TNF-a, TGF-ß and osteopontin became upregulated. The results indicate that biomaterial surface texturing, especially at the nanometric scale, can be used to control macrophage activation to induce a wound healing response, rather than a profound inflammatory response.
Originele taal-2Engels
Pagina's (van-tot)308-317
TijdschriftNanomedicine
Volume8
Nummer van het tijdschrift3
DOI's
StatusGepubliceerd - 2012

Vingerafdruk

Macrophages
Biocompatible Materials
Interleukin-1
Cytokines
Biomaterials
Osteopontin
Macrophage Activation
Substrates
Cell Adhesion
Wound Healing
Extracellular Matrix
Lasers
Cell Culture Techniques
Texturing
Cell adhesion
Reactive ion etching
Ions
Cell culture
Ion implantation
Lithography

Citeer dit

Lamers, E., Walboomers, X. F., Domanski, M., Prodanov, L., Melis, J., Luttge, R., ... Jansen, J. A. (2012). In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates. Nanomedicine, 8(3), 308-317. https://doi.org/10.1016/j.nano.2011.06.013
Lamers, E. ; Walboomers, X.F. ; Domanski, M. ; Prodanov, L. ; Melis, J. ; Luttge, R. ; Winnubst, A.J.A. ; Anderson, J.M. ; Gardeniers, J.G.E. ; Jansen, J.A. / In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates. In: Nanomedicine. 2012 ; Vol. 8, Nr. 3. blz. 308-317.
@article{f315ed84e76246eab578b87f202d878a,
title = "In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates",
abstract = "The immune response to an implanted biomaterial is orchestrated by macrophages. In this study various nanogrooved patterns were created by using laser interference lithography and reactive ion etching. The created nanogrooves mimic the natural extracellular matrix environment. Macrophage cell culture demonstrated that interleukin 1{\ss} and TNF-a cytokine production were upregulated on nanogrooved substrates. In vivo subcutaneous implantation in a validated mouse cage model for 14 days demonstrated that nanogrooves enhanced and guided cell adhesion, and few multinucleated cells were formed. In agreement with the in vitro results, cytokine production was found to be nanogroove dependent, as interleukin 1{\ss}, TNF-a, TGF-{\ss} and osteopontin became upregulated. The results indicate that biomaterial surface texturing, especially at the nanometric scale, can be used to control macrophage activation to induce a wound healing response, rather than a profound inflammatory response.",
author = "E. Lamers and X.F. Walboomers and M. Domanski and L. Prodanov and J. Melis and R. Luttge and A.J.A. Winnubst and J.M. Anderson and J.G.E. Gardeniers and J.A. Jansen",
year = "2012",
doi = "10.1016/j.nano.2011.06.013",
language = "English",
volume = "8",
pages = "308--317",
journal = "Nanomedicine",
issn = "1743-5889",
publisher = "Future Medicine Ltd.",
number = "3",

}

Lamers, E, Walboomers, XF, Domanski, M, Prodanov, L, Melis, J, Luttge, R, Winnubst, AJA, Anderson, JM, Gardeniers, JGE & Jansen, JA 2012, 'In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates', Nanomedicine, vol. 8, nr. 3, blz. 308-317. https://doi.org/10.1016/j.nano.2011.06.013

In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates. / Lamers, E.; Walboomers, X.F.; Domanski, M.; Prodanov, L.; Melis, J.; Luttge, R.; Winnubst, A.J.A.; Anderson, J.M.; Gardeniers, J.G.E.; Jansen, J.A.

In: Nanomedicine, Vol. 8, Nr. 3, 2012, blz. 308-317.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

TY - JOUR

T1 - In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates

AU - Lamers, E.

AU - Walboomers, X.F.

AU - Domanski, M.

AU - Prodanov, L.

AU - Melis, J.

AU - Luttge, R.

AU - Winnubst, A.J.A.

AU - Anderson, J.M.

AU - Gardeniers, J.G.E.

AU - Jansen, J.A.

PY - 2012

Y1 - 2012

N2 - The immune response to an implanted biomaterial is orchestrated by macrophages. In this study various nanogrooved patterns were created by using laser interference lithography and reactive ion etching. The created nanogrooves mimic the natural extracellular matrix environment. Macrophage cell culture demonstrated that interleukin 1ß and TNF-a cytokine production were upregulated on nanogrooved substrates. In vivo subcutaneous implantation in a validated mouse cage model for 14 days demonstrated that nanogrooves enhanced and guided cell adhesion, and few multinucleated cells were formed. In agreement with the in vitro results, cytokine production was found to be nanogroove dependent, as interleukin 1ß, TNF-a, TGF-ß and osteopontin became upregulated. The results indicate that biomaterial surface texturing, especially at the nanometric scale, can be used to control macrophage activation to induce a wound healing response, rather than a profound inflammatory response.

AB - The immune response to an implanted biomaterial is orchestrated by macrophages. In this study various nanogrooved patterns were created by using laser interference lithography and reactive ion etching. The created nanogrooves mimic the natural extracellular matrix environment. Macrophage cell culture demonstrated that interleukin 1ß and TNF-a cytokine production were upregulated on nanogrooved substrates. In vivo subcutaneous implantation in a validated mouse cage model for 14 days demonstrated that nanogrooves enhanced and guided cell adhesion, and few multinucleated cells were formed. In agreement with the in vitro results, cytokine production was found to be nanogroove dependent, as interleukin 1ß, TNF-a, TGF-ß and osteopontin became upregulated. The results indicate that biomaterial surface texturing, especially at the nanometric scale, can be used to control macrophage activation to induce a wound healing response, rather than a profound inflammatory response.

U2 - 10.1016/j.nano.2011.06.013

DO - 10.1016/j.nano.2011.06.013

M3 - Article

C2 - 21704595

VL - 8

SP - 308

EP - 317

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 3

ER -