Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials

Hanna Talacua; Serge H M Söntjens; Shraddha H Thakkar; Aurelie M A Brizard; Lex A van Herwerden; Aryan Vink; Geert C van Almen; Patricia Y W Dankers; Carlijn V C Bouten; Ricardo P J Budde; Henk M Janssen; Jolanda Kluin

doi:10.1002/mabi.202000024

Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials

Hanna Talacua, Serge H M Söntjens, Shraddha H Thakkar, Aurelie M A Brizard, Lex A van Herwerden, Aryan Vink, Geert C van Almen, Patricia Y W Dankers (Corresponding author), Carlijn V C Bouten, Ricardo P J Budde, Henk M Janssen, Jolanda Kluin

Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review

11 Citaten (Scopus)

Samenvatting

For in situ tissue engineering (TE) applications it is important that implant degradation proceeds in concord with neo-tissue formation to avoid graft failure. It will therefore be valuable to have an imaging contrast agent (CA) available that can report on the degrading implant. For this purpose, a biodegradable radiopaque biomaterial is presented, modularly composed of a bisurea chain-extended polycaprolactone (PCL2000-U4U) elastomer and a novel iodinated bisurea-modified CA additive (I-U4U). Supramolecular hydrogen bonding interactions between the components ensure their intimate mixing. Porous implant TE-grafts are prepared by simply electrospinning a solution containing PCL2000-U4U and I-U4U. Rats receive an aortic interposition graft, either composed of only PCL2000-U4U (control) or of PCL2000-U4U and I-U4U (test). The grafts are explanted for analysis at three time points over a 1-month period. Computed tomography imaging of the test group implants prior to explantation shows a decrease in iodide volume and density over time. Explant analysis also indicates scaffold degradation. (Immuno)histochemistry shows comparable cellular contents and a similar neo-tissue formation process for test and control group, demonstrating that the CA does not have apparent adverse effects. A supramolecular approach to create solid radiopaque biomaterials can therefore be used to noninvasively monitor the biodegradation of synthetic implants.

Originele taal-2	Engels
Artikelnummer	2000024
Aantal pagina's	14
Tijdschrift	Macromolecular Bioscience
Volume	20
Nummer van het tijdschrift	7
Vroegere onlinedatum	17 jun. 2020
DOI's	https://doi.org/10.1002/mabi.202000024
Status	Gepubliceerd - 1 jul. 2020

Bibliografische nota

Financiering

This research formed part of the iValve project of the research program of the BioMedical Materials institute, co-founded by the Dutch Ministry of Economic Affairs, Agriculture and Innovation. The financial contribution of the Nederlandse Hartstichting is most gratefully acknowledged. All members of the iValve project are thanked for valuable discussions. Joost L.J. van Dongen, Xianwen Lou (from the Macromolecular and Organic Chemistry group at the TU/e Eindhoven, the Netherlands) and Michel Fransen (SyMO-Chem BV) are thanked for their contributions and help on MS (HRMS, MALDI-TOF-MS, HPLC-MS and GC-MS) and GPC measurements. This research formed part of the iValve project of the research program of the BioMedical Materials institute, co‐founded by the Dutch Ministry of Economic Affairs, Agriculture and Innovation. The financial contribution of the Nederlandse Hartstichting is most gratefully acknowledged. All members of the iValve project are thanked for valuable discussions. Joost L.J. van Dongen, Xianwen Lou (from the Macromolecular and Organic Chemistry group at the TU/e Eindhoven, the Netherlands) and Michel Fransen (SyMO‐Chem BV) are thanked for their contributions and help on MS (HRMS, MALDI‐TOF‐MS, HPLC‐MS and GC‐MS) and GPC measurements.

Financiers	Financiernummer
BioMedical Materials institute
Dutch Ministry of Economic Affairs, Agriculture and Innovation
Ministerie van Economische Zaken en Klimaat

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10.1002/mabi.202000024

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Talacua, H., Söntjens, S. H. M., Thakkar, S. H., Brizard, A. M. A., van Herwerden, L. A., Vink, A., van Almen, G. C., Dankers, P. Y. W., Bouten, C. V. C., Budde, R. P. J., Janssen, H. M., & Kluin, J. (2020). Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials. Macromolecular Bioscience, 20(7), Artikel 2000024. https://doi.org/10.1002/mabi.202000024

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title = "Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials",

abstract = "For in situ tissue engineering (TE) applications it is important that implant degradation proceeds in concord with neo-tissue formation to avoid graft failure. It will therefore be valuable to have an imaging contrast agent (CA) available that can report on the degrading implant. For this purpose, a biodegradable radiopaque biomaterial is presented, modularly composed of a bisurea chain-extended polycaprolactone (PCL2000-U4U) elastomer and a novel iodinated bisurea-modified CA additive (I-U4U). Supramolecular hydrogen bonding interactions between the components ensure their intimate mixing. Porous implant TE-grafts are prepared by simply electrospinning a solution containing PCL2000-U4U and I-U4U. Rats receive an aortic interposition graft, either composed of only PCL2000-U4U (control) or of PCL2000-U4U and I-U4U (test). The grafts are explanted for analysis at three time points over a 1-month period. Computed tomography imaging of the test group implants prior to explantation shows a decrease in iodide volume and density over time. Explant analysis also indicates scaffold degradation. (Immuno)histochemistry shows comparable cellular contents and a similar neo-tissue formation process for test and control group, demonstrating that the CA does not have apparent adverse effects. A supramolecular approach to create solid radiopaque biomaterials can therefore be used to noninvasively monitor the biodegradation of synthetic implants.",

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author = "Hanna Talacua and S{\"o}ntjens, {Serge H M} and Thakkar, {Shraddha H} and Brizard, {Aurelie M A} and {van Herwerden}, {Lex A} and Aryan Vink and {van Almen}, {Geert C} and Dankers, {Patricia Y W} and Bouten, {Carlijn V C} and Budde, {Ricardo P J} and Janssen, {Henk M} and Jolanda Kluin",

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Talacua, H, Söntjens, SHM, Thakkar, SH, Brizard, AMA, van Herwerden, LA, Vink, A, van Almen, GC, Dankers, PYW , Bouten, CVC, Budde, RPJ, Janssen, HM & Kluin, J 2020, 'Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials', Macromolecular Bioscience, vol. 20, nr. 7, 2000024. https://doi.org/10.1002/mabi.202000024

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AU - Talacua, Hanna

AU - Söntjens, Serge H M

AU - Thakkar, Shraddha H

AU - Brizard, Aurelie M A

AU - van Herwerden, Lex A

AU - Vink, Aryan

AU - van Almen, Geert C

AU - Dankers, Patricia Y W

AU - Bouten, Carlijn V C

AU - Budde, Ricardo P J

AU - Janssen, Henk M

AU - Kluin, Jolanda

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N2 - For in situ tissue engineering (TE) applications it is important that implant degradation proceeds in concord with neo-tissue formation to avoid graft failure. It will therefore be valuable to have an imaging contrast agent (CA) available that can report on the degrading implant. For this purpose, a biodegradable radiopaque biomaterial is presented, modularly composed of a bisurea chain-extended polycaprolactone (PCL2000-U4U) elastomer and a novel iodinated bisurea-modified CA additive (I-U4U). Supramolecular hydrogen bonding interactions between the components ensure their intimate mixing. Porous implant TE-grafts are prepared by simply electrospinning a solution containing PCL2000-U4U and I-U4U. Rats receive an aortic interposition graft, either composed of only PCL2000-U4U (control) or of PCL2000-U4U and I-U4U (test). The grafts are explanted for analysis at three time points over a 1-month period. Computed tomography imaging of the test group implants prior to explantation shows a decrease in iodide volume and density over time. Explant analysis also indicates scaffold degradation. (Immuno)histochemistry shows comparable cellular contents and a similar neo-tissue formation process for test and control group, demonstrating that the CA does not have apparent adverse effects. A supramolecular approach to create solid radiopaque biomaterials can therefore be used to noninvasively monitor the biodegradation of synthetic implants.

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Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials

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