Hydrogel/poly-dimethylsiloxane hybrid bioreactor facilitating 3D cell culturing

B. Schurink, R. Luttge

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The authors present a hydrogel/poly-dimethylsiloxane (PDMS) hybrid bioreactor. The bioreactor enables a low shear stress 3D culture by integrating a hydrogel as a barrier into a PDMS casing. The use of PDMS allows the reversible adhesion of the device to a commercially available microelectrode array. A two-step molding process facilitates this relatively simple, cost effective, and leakage-free add-on microculture system. Agarose (2%) is used as hydrogel barrier material and mass transport is evaluated by fluorescein isothiocyanate-albumin fluorescence under static conditions which yields a diffusion coefficient of average value of 2.2 × 10-7 cm 2 s-1 across the barrier. To validate our bioreactor for diffusion limited 3D cell culture, rat cortical cells were successfully cultured in Matrigel for 6 days. © 2013 American Vacuum Society.
Originele taal-2Engels
Artikelnummer06F903
Aantal pagina's5
TijdschriftJournal of Vacuum Science and Technology, B
Volume31
Nummer van het tijdschrift6
DOI's
StatusGepubliceerd - 2013

Vingerafdruk

cell culturing
bioreactors
Polydimethylsiloxane
Bioreactors
Hydrogels
casing
Microelectrodes
albumins
Cell culture
Molding
shear stress
rats
Rats
Shear stress
adhesion
leakage
Adhesion
Mass transfer
diffusion coefficient
Fluorescence

Citeer dit

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abstract = "The authors present a hydrogel/poly-dimethylsiloxane (PDMS) hybrid bioreactor. The bioreactor enables a low shear stress 3D culture by integrating a hydrogel as a barrier into a PDMS casing. The use of PDMS allows the reversible adhesion of the device to a commercially available microelectrode array. A two-step molding process facilitates this relatively simple, cost effective, and leakage-free add-on microculture system. Agarose (2{\%}) is used as hydrogel barrier material and mass transport is evaluated by fluorescein isothiocyanate-albumin fluorescence under static conditions which yields a diffusion coefficient of average value of 2.2 × 10-7 cm 2 s-1 across the barrier. To validate our bioreactor for diffusion limited 3D cell culture, rat cortical cells were successfully cultured in Matrigel for 6 days. {\circledC} 2013 American Vacuum Society.",
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Hydrogel/poly-dimethylsiloxane hybrid bioreactor facilitating 3D cell culturing. / Schurink, B.; Luttge, R.

In: Journal of Vacuum Science and Technology, B, Vol. 31, Nr. 6, 06F903, 2013.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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