Functional peptide presentation on different hydrogen bonding biomaterials using supramolecular additives

Ronald C. van Gaal, Antonetta B.C. Buskermolen, Bastiaan D. Ippel, Peter-Paul K.H. Fransen, Sabrina Zaccaria, Carlijn V.C. Bouten, Patricia Y.W. Dankers (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

1 Downloads (Pure)

Uittreksel

Supramolecular biomaterials based on hydrogen bonding units can be conveniently functionalized in a mix-and-match approach using supramolecular additives. The presentation of bioactive additives has been sparsely investigated in supramolecular-based elastomeric biomaterials. Here it was investigated how cell adhesive peptides are presented and affect the surface in supramolecular biomaterials based either on ureido-pyrimidinone (UPy) or bisurea (BU) moieties. Polycaprolactone modified with UPy or BU moieties served as the base material. RGD or cyclic (c)RGD were conjugated to complementary supramolecular motifs, and were mixed with the corresponding base materials as supramolecular additives. Biomaterial surface morphology changed upon bioactivation, resulting in the formation of random aggregates on UPy-based materials, and fibrous aggregates on BU-materials. Moreover, peptide type affected aggregation morphology, in which RGD led to larger cluster formation than cRGD. Increased cRGD concentrations led to reduced focal adhesion size and cell migration velocity, and increased focal adhesion numbers in both systems, yet most prominent on functionalized BU-biomaterials. In conclusion, both systems exhibited distinct peptide presenting properties, of which the BU-system most strongly affected cellular adhesive behavior on the biomaterial. This research provided deeper insights in the differences between supramolecular elastomeric platforms, and the level of peptide introduction for biomaterial applications.

Originele taal-2Engels
Artikelnummer119466
Aantal pagina's9
TijdschriftBiomaterials
Volume224
DOI's
StatusGepubliceerd - dec 2019

Vingerafdruk

Biocompatible Materials
Hydrogen Bonding
Biomaterials
Peptides
Hydrogen bonds
Pyrimidinones
Focal Adhesions
Adhesives
Adhesion
Polycaprolactone
Cell Movement
Surface morphology
Agglomeration
Research

Citeer dit

@article{bce1c6a2a0874e238081a0c8f526cc3e,
title = "Functional peptide presentation on different hydrogen bonding biomaterials using supramolecular additives",
abstract = "Supramolecular biomaterials based on hydrogen bonding units can be conveniently functionalized in a mix-and-match approach using supramolecular additives. The presentation of bioactive additives has been sparsely investigated in supramolecular-based elastomeric biomaterials. Here it was investigated how cell adhesive peptides are presented and affect the surface in supramolecular biomaterials based either on ureido-pyrimidinone (UPy) or bisurea (BU) moieties. Polycaprolactone modified with UPy or BU moieties served as the base material. RGD or cyclic (c)RGD were conjugated to complementary supramolecular motifs, and were mixed with the corresponding base materials as supramolecular additives. Biomaterial surface morphology changed upon bioactivation, resulting in the formation of random aggregates on UPy-based materials, and fibrous aggregates on BU-materials. Moreover, peptide type affected aggregation morphology, in which RGD led to larger cluster formation than cRGD. Increased cRGD concentrations led to reduced focal adhesion size and cell migration velocity, and increased focal adhesion numbers in both systems, yet most prominent on functionalized BU-biomaterials. In conclusion, both systems exhibited distinct peptide presenting properties, of which the BU-system most strongly affected cellular adhesive behavior on the biomaterial. This research provided deeper insights in the differences between supramolecular elastomeric platforms, and the level of peptide introduction for biomaterial applications.",
author = "{van Gaal}, {Ronald C.} and Buskermolen, {Antonetta B.C.} and Ippel, {Bastiaan D.} and Fransen, {Peter-Paul K.H.} and Sabrina Zaccaria and Bouten, {Carlijn V.C.} and Dankers, {Patricia Y.W.}",
note = "Copyright {\circledC} 2019 Elsevier Ltd. All rights reserved.",
year = "2019",
month = "12",
doi = "10.1016/j.biomaterials.2019.119466",
language = "English",
volume = "224",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",

}

Functional peptide presentation on different hydrogen bonding biomaterials using supramolecular additives. / van Gaal, Ronald C.; Buskermolen, Antonetta B.C.; Ippel, Bastiaan D.; Fransen, Peter-Paul K.H.; Zaccaria, Sabrina; Bouten, Carlijn V.C.; Dankers, Patricia Y.W. (Corresponding author).

In: Biomaterials, Vol. 224, 119466, 12.2019.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

TY - JOUR

T1 - Functional peptide presentation on different hydrogen bonding biomaterials using supramolecular additives

AU - van Gaal, Ronald C.

AU - Buskermolen, Antonetta B.C.

AU - Ippel, Bastiaan D.

AU - Fransen, Peter-Paul K.H.

AU - Zaccaria, Sabrina

AU - Bouten, Carlijn V.C.

AU - Dankers, Patricia Y.W.

N1 - Copyright © 2019 Elsevier Ltd. All rights reserved.

PY - 2019/12

Y1 - 2019/12

N2 - Supramolecular biomaterials based on hydrogen bonding units can be conveniently functionalized in a mix-and-match approach using supramolecular additives. The presentation of bioactive additives has been sparsely investigated in supramolecular-based elastomeric biomaterials. Here it was investigated how cell adhesive peptides are presented and affect the surface in supramolecular biomaterials based either on ureido-pyrimidinone (UPy) or bisurea (BU) moieties. Polycaprolactone modified with UPy or BU moieties served as the base material. RGD or cyclic (c)RGD were conjugated to complementary supramolecular motifs, and were mixed with the corresponding base materials as supramolecular additives. Biomaterial surface morphology changed upon bioactivation, resulting in the formation of random aggregates on UPy-based materials, and fibrous aggregates on BU-materials. Moreover, peptide type affected aggregation morphology, in which RGD led to larger cluster formation than cRGD. Increased cRGD concentrations led to reduced focal adhesion size and cell migration velocity, and increased focal adhesion numbers in both systems, yet most prominent on functionalized BU-biomaterials. In conclusion, both systems exhibited distinct peptide presenting properties, of which the BU-system most strongly affected cellular adhesive behavior on the biomaterial. This research provided deeper insights in the differences between supramolecular elastomeric platforms, and the level of peptide introduction for biomaterial applications.

AB - Supramolecular biomaterials based on hydrogen bonding units can be conveniently functionalized in a mix-and-match approach using supramolecular additives. The presentation of bioactive additives has been sparsely investigated in supramolecular-based elastomeric biomaterials. Here it was investigated how cell adhesive peptides are presented and affect the surface in supramolecular biomaterials based either on ureido-pyrimidinone (UPy) or bisurea (BU) moieties. Polycaprolactone modified with UPy or BU moieties served as the base material. RGD or cyclic (c)RGD were conjugated to complementary supramolecular motifs, and were mixed with the corresponding base materials as supramolecular additives. Biomaterial surface morphology changed upon bioactivation, resulting in the formation of random aggregates on UPy-based materials, and fibrous aggregates on BU-materials. Moreover, peptide type affected aggregation morphology, in which RGD led to larger cluster formation than cRGD. Increased cRGD concentrations led to reduced focal adhesion size and cell migration velocity, and increased focal adhesion numbers in both systems, yet most prominent on functionalized BU-biomaterials. In conclusion, both systems exhibited distinct peptide presenting properties, of which the BU-system most strongly affected cellular adhesive behavior on the biomaterial. This research provided deeper insights in the differences between supramolecular elastomeric platforms, and the level of peptide introduction for biomaterial applications.

U2 - 10.1016/j.biomaterials.2019.119466

DO - 10.1016/j.biomaterials.2019.119466

M3 - Article

C2 - 31542516

VL - 224

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

M1 - 119466

ER -