Fluorescent imaging of transition metal homeostasis using genetically encoded sensors

J.L. Vinkenborg, M.S.T. Koay, M. Merkx

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

61 Citaten (Scopus)
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Samenvatting

The ability to image the concentration of transition metals in living cells in real time is important for understanding transition metal (TM) homeostasis and its involvement in diseases. Genetically encoded fluorescent sensor proteins are attractive because they do not require cell-invasive procedures, can be targeted to different locations in the cell, and allow ratiometric detection. Important progress in the development of Zn2+ sensors has allowed sensitive detection of the very low free concentrations of Zn2+ in single cells, both in the cytosol and various organelles. Together with other recent advances in chemical biology, these tools seem particularly useful to interrogate the dynamics and compartmentation of TM homeostasis.
Originele taal-2Engels
Pagina's (van-tot)231-237
Aantal pagina's7
TijdschriftCurrent Opinion in Chemical Biology
Volume14
Nummer van het tijdschrift2
DOI's
StatusGepubliceerd - 2010

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