Uittreksel
In biology, a variety of highly ordered nanometer-size protein cages is found. Such structures find increasing application in, for example, vaccination, drug delivery, and catalysis. Understanding the physiochemical properties, particularly inside the confinement of a protein cage, helps to predict the behavior and properties of new materials based on such particles. Here, the relation between the bulk solution pH and the local pH inside a model protein cage, based on virus-like particles (VLPs) built from the coat proteins of the cowpea chlorotic mottle virus, is investigated. The pH is a crucial parameter in a variety of processes and is potentially significantly influenced by the high concentration of charges residing on the interior of the VLPs. The data show a systematic more acidic pH of 0.5 unit inside the VLP compared to that of the bulk solution for pH values above pH 6, which is explained using a theoretical model based on a Donnan equilibrium. The model agrees with the experimental data over almost two orders of magnitude, while below pH 6 the experimental data point to a buffering capacity of the VLP. These results are a first step in a better understanding of the physiochemical conditions inside a protein cage.
| Taal | Engels |
|---|---|
| Artikelnummer | 1802081 |
| Aantal pagina's | 7 |
| Tijdschrift | Small |
| Volume | 14 |
| Nummer van het tijdschrift | 36 |
| DOI's | |
| Status | Gepubliceerd - 6 sep 2018 |
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Experimental and theoretical determination of the pH inside the confinement of a virus-like particle. / Maassen, Stan J.; van der Schoot, Paul; Cornelissen, Jeroen J.L.M.
In: Small, Vol. 14, Nr. 36, 1802081, 06.09.2018.Onderzoeksoutput: Bijdrage aan tijdschrift › Tijdschriftartikel › Academic › peer review
TY - JOUR
T1 - Experimental and theoretical determination of the pH inside the confinement of a virus-like particle
AU - Maassen,Stan J.
AU - van der Schoot,Paul
AU - Cornelissen,Jeroen J.L.M.
PY - 2018/9/6
Y1 - 2018/9/6
N2 - In biology, a variety of highly ordered nanometer-size protein cages is found. Such structures find increasing application in, for example, vaccination, drug delivery, and catalysis. Understanding the physiochemical properties, particularly inside the confinement of a protein cage, helps to predict the behavior and properties of new materials based on such particles. Here, the relation between the bulk solution pH and the local pH inside a model protein cage, based on virus-like particles (VLPs) built from the coat proteins of the cowpea chlorotic mottle virus, is investigated. The pH is a crucial parameter in a variety of processes and is potentially significantly influenced by the high concentration of charges residing on the interior of the VLPs. The data show a systematic more acidic pH of 0.5 unit inside the VLP compared to that of the bulk solution for pH values above pH 6, which is explained using a theoretical model based on a Donnan equilibrium. The model agrees with the experimental data over almost two orders of magnitude, while below pH 6 the experimental data point to a buffering capacity of the VLP. These results are a first step in a better understanding of the physiochemical conditions inside a protein cage.
AB - In biology, a variety of highly ordered nanometer-size protein cages is found. Such structures find increasing application in, for example, vaccination, drug delivery, and catalysis. Understanding the physiochemical properties, particularly inside the confinement of a protein cage, helps to predict the behavior and properties of new materials based on such particles. Here, the relation between the bulk solution pH and the local pH inside a model protein cage, based on virus-like particles (VLPs) built from the coat proteins of the cowpea chlorotic mottle virus, is investigated. The pH is a crucial parameter in a variety of processes and is potentially significantly influenced by the high concentration of charges residing on the interior of the VLPs. The data show a systematic more acidic pH of 0.5 unit inside the VLP compared to that of the bulk solution for pH values above pH 6, which is explained using a theoretical model based on a Donnan equilibrium. The model agrees with the experimental data over almost two orders of magnitude, while below pH 6 the experimental data point to a buffering capacity of the VLP. These results are a first step in a better understanding of the physiochemical conditions inside a protein cage.
KW - confinement
KW - nanotechnology
KW - pH measurements
KW - physical chemistry
KW - virus capsids
UR - http://www.scopus.com/inward/record.url?scp=85052663179&partnerID=8YFLogxK
U2 - 10.1002/smll.201802081
DO - 10.1002/smll.201802081
M3 - Article
VL - 14
JO - Small
T2 - Small
JF - Small
SN - 1613-6810
IS - 36
M1 - 1802081
ER -