Enhanced fluorescence of a dye on DNA-assembled gold nanodimers discriminated by lifetime correlation spectroscopy

Pedro M.R. Paulo, David Botequim, Agnieszka Jóskowiak, Sofia Martins, Duarte M.F. Prazeres, Peter Zijlstra, Sílvia M.B. Costa

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1 Citaat (Scopus)

Uittreksel

The surface plasmon modes of metal nanoparticles provide a way to efficiently enhance the excitation and emission from a fluorescent dye. We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon-enhanced fluorescence of this dye using dimers of 80 nm particles was measured at the single-molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from nonenhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. Indeed, the frequency of enhancement events is larger than that expected from the volume occupancy of the gap region, thus suggesting that the interaction of the dye with DNA linkers favors the observation of emission enhancement in our dimer particles.

TaalEngels
Pagina's10971-10980
Aantal pagina's10
TijdschriftJournal of Physical Chemistry C
Volume122
Nummer van het tijdschrift20
DOI's
StatusGepubliceerd - 24 mei 2018

Vingerafdruk

Gold
Dimers
DNA
Coloring Agents
Dyes
deoxyribonucleic acid
dyes
Fluorescence
dimers
Spectroscopy
gold
life (durability)
fluorescence
spectroscopy
augmentation
nanoparticles
Metal nanoparticles
Fluorescent Dyes
assembly
interactions

Citeer dit

Paulo, P. M. R., Botequim, D., Jóskowiak, A., Martins, S., Prazeres, D. M. F., Zijlstra, P., & Costa, S. M. B. (2018). Enhanced fluorescence of a dye on DNA-assembled gold nanodimers discriminated by lifetime correlation spectroscopy. Journal of Physical Chemistry C, 122(20), 10971-10980. DOI: 10.1021/acs.jpcc.7b12622
Paulo, Pedro M.R. ; Botequim, David ; Jóskowiak, Agnieszka ; Martins, Sofia ; Prazeres, Duarte M.F. ; Zijlstra, Peter ; Costa, Sílvia M.B./ Enhanced fluorescence of a dye on DNA-assembled gold nanodimers discriminated by lifetime correlation spectroscopy. In: Journal of Physical Chemistry C. 2018 ; Vol. 122, Nr. 20. blz. 10971-10980
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abstract = "The surface plasmon modes of metal nanoparticles provide a way to efficiently enhance the excitation and emission from a fluorescent dye. We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon-enhanced fluorescence of this dye using dimers of 80 nm particles was measured at the single-molecule detection level. The top enhancement factors were above 1000-fold in 71{\%} of the dimers within a total of 32 dimers measured, and in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from nonenhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. Indeed, the frequency of enhancement events is larger than that expected from the volume occupancy of the gap region, thus suggesting that the interaction of the dye with DNA linkers favors the observation of emission enhancement in our dimer particles.",
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Paulo, PMR, Botequim, D, Jóskowiak, A, Martins, S, Prazeres, DMF, Zijlstra, P & Costa, SMB 2018, 'Enhanced fluorescence of a dye on DNA-assembled gold nanodimers discriminated by lifetime correlation spectroscopy' Journal of Physical Chemistry C, vol. 122, nr. 20, blz. 10971-10980. DOI: 10.1021/acs.jpcc.7b12622

Enhanced fluorescence of a dye on DNA-assembled gold nanodimers discriminated by lifetime correlation spectroscopy. / Paulo, Pedro M.R.; Botequim, David; Jóskowiak, Agnieszka; Martins, Sofia; Prazeres, Duarte M.F.; Zijlstra, Peter; Costa, Sílvia M.B.

In: Journal of Physical Chemistry C, Vol. 122, Nr. 20, 24.05.2018, blz. 10971-10980.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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AU - Jóskowiak,Agnieszka

AU - Martins,Sofia

AU - Prazeres,Duarte M.F.

AU - Zijlstra,Peter

AU - Costa,Sílvia M.B.

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N2 - The surface plasmon modes of metal nanoparticles provide a way to efficiently enhance the excitation and emission from a fluorescent dye. We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon-enhanced fluorescence of this dye using dimers of 80 nm particles was measured at the single-molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from nonenhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. Indeed, the frequency of enhancement events is larger than that expected from the volume occupancy of the gap region, thus suggesting that the interaction of the dye with DNA linkers favors the observation of emission enhancement in our dimer particles.

AB - The surface plasmon modes of metal nanoparticles provide a way to efficiently enhance the excitation and emission from a fluorescent dye. We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon-enhanced fluorescence of this dye using dimers of 80 nm particles was measured at the single-molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from nonenhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. Indeed, the frequency of enhancement events is larger than that expected from the volume occupancy of the gap region, thus suggesting that the interaction of the dye with DNA linkers favors the observation of emission enhancement in our dimer particles.

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Paulo PMR, Botequim D, Jóskowiak A, Martins S, Prazeres DMF, Zijlstra P et al. Enhanced fluorescence of a dye on DNA-assembled gold nanodimers discriminated by lifetime correlation spectroscopy. Journal of Physical Chemistry C. 2018 mei 24;122(20):10971-10980. Beschikbaar vanaf, DOI: 10.1021/acs.jpcc.7b12622