TY - JOUR
T1 - Engineering with NanoLuc
T2 - A playground for the development of bioluminescent protein switches and sensors
AU - Biewenga, Lieuwe
AU - Rosier, Bas J.H.M.
AU - Merkx, Maarten
PY - 2020/12/18
Y1 - 2020/12/18
N2 - The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability. The continuously expanding NanoLuc toolbox has been employed in applications ranging from biosensors to molecular and cellular imaging, and currently includes split complementation variants, engineering techniques for spectral tuning, and bioluminescence resonance energy transfer-based concepts. In this review, we provide an overview of state-of-the-art NanoLuc-based sensors and switches with a focus on the underlying protein engineering approaches. We discuss the advantages and disadvantages of various strategies with respect to sensor sensitivity, modularity, and dynamic range of the sensor and provide a perspective on future strategies and applications.
AB - The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability. The continuously expanding NanoLuc toolbox has been employed in applications ranging from biosensors to molecular and cellular imaging, and currently includes split complementation variants, engineering techniques for spectral tuning, and bioluminescence resonance energy transfer-based concepts. In this review, we provide an overview of state-of-the-art NanoLuc-based sensors and switches with a focus on the underlying protein engineering approaches. We discuss the advantages and disadvantages of various strategies with respect to sensor sensitivity, modularity, and dynamic range of the sensor and provide a perspective on future strategies and applications.
UR - http://www.scopus.com/inward/record.url?scp=85097894697&partnerID=8YFLogxK
U2 - 10.1042/BST20200440
DO - 10.1042/BST20200440
M3 - Review article
C2 - 33242085
AN - SCOPUS:85097894697
SN - 0300-5127
VL - 48
SP - 2643
EP - 2655
JO - Biochemical Society Transactions
JF - Biochemical Society Transactions
IS - 6
ER -