Samenvatting
The macromolecule-mediated binding of particles to a surface is often characterized by association and dissociation rates. These have mostly been modeled as thermally activated processes governed by a single energy barrier. However, our magnetic tweezers experiments show that the single energy barrier description is not generally applicable. To take account of interactions with an inhomogeneous chemical or physical origin, we introduce a distribution of energy barriers. Association experiments under a rotating magnetic field [Kemper, Langmuir 2012] are interpreted using a parameterization of the barrier as a function of ionic strength. The distribution of energy barriers is derived from experiments with and without protein-specific interactions. For dissociation experiments under translational forces, the measured time-dependent curves are well reproduced by the model. These biophysical insights are important for the development of novel particle-based lab-on-chip biosensing systems.
Originele taal-2 | Engels |
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Status | Gepubliceerd - 2013 |
Evenement | Physics@FOM Veldhoven 2013 - Koningshof, Veldhoven, Nederland Duur: 22 jan. 2013 → 23 jan. 2013 |
Congres
Congres | Physics@FOM Veldhoven 2013 |
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Land/Regio | Nederland |
Stad | Veldhoven |
Periode | 22/01/13 → 23/01/13 |
Ander | FOM Physics 2013, Veldhoven Koningshof |