Effects of artepillin C on model membranes displaying liquid immiscibility

W.M. Pazin (Corresponding author), N. Vilanova, I.K. Voets, A.E.E. Soares, A.S. Ito

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

Uittreksel

It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and giant unilamelar vesicles) composed of ternary lipid mixtures containing cholesterol, which display liquid-ordered (lo) and liquid-disordered (ld) phase coexistence. Specifically, we explored potential changes in relevant membrane parameters upon addition of artepillin C presenting both neutral and deprotonated states by means of small angle X-ray scattering (SAXS), differential scanning calorimetry (DSC), and confocal and multiphoton excitation fluorescence microscopy. Thermotropic analysis obtained from DSC experiments indicated a loss in the lipid cooperativity of lo phase at equilibrium conditions, while at similar conditions spontaneous formation of unilamellar vesicles from SAXS experiments showed that deprotonated artepillin C preferentially located at the surface of the membrane. Time-resolved experiments using fluorescence microscopy showed that at doses above 100 µM, artepillin C in its neutral state interacted with both liquid-ordered and liquid-disordered phases, inducing curvature stress and promoting dehydration at the membrane interface.

TaalEngels
Pagina'se8281
Aantal pagina's11
TijdschriftBrazilian Journal of Medical and Biological Research
Volume52
Nummer van het tijdschrift3
DOI's
StatusGepubliceerd - 25 mrt 2019

Vingerafdruk

Liquid membranes
Solubility
Membranes
Liquids
Propolis
Fluorescence microscopy
Differential Scanning Calorimetry
X ray scattering
Multiphoton Fluorescence Microscopy
Differential scanning calorimetry
X-Rays
Biological membranes
Lipids
Unilamellar Liposomes
Lipid bilayers
Experiments
Lipid Bilayers
Therapeutic Uses
Dehydration
Fluorescence Microscopy

Citeer dit

Pazin, W.M. ; Vilanova, N. ; Voets, I.K. ; Soares, A.E.E. ; Ito, A.S./ Effects of artepillin C on model membranes displaying liquid immiscibility. In: Brazilian Journal of Medical and Biological Research. 2019 ; Vol. 52, Nr. 3. blz. e8281
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Effects of artepillin C on model membranes displaying liquid immiscibility. / Pazin, W.M. (Corresponding author); Vilanova, N.; Voets, I.K.; Soares, A.E.E.; Ito, A.S.

In: Brazilian Journal of Medical and Biological Research, Vol. 52, Nr. 3, 25.03.2019, blz. e8281.

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

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