Dynamic protease activation on a multimeric synthetic protein scaffold via adaptable DNA-based recruitment domains

Tsuyoshi Mashima, Bas J.H.M. Rosier, Koji Oohora, Tom F.A. de Greef, Takashi Hayashi, Luc Brunsveld (Corresponding author)

Onderzoeksoutput: Bijdrage aan tijdschriftTijdschriftartikelAcademicpeer review

Samenvatting

Hexameric hemoprotein (HTHP) is employed as a scaffold protein for the supramolecular assembly and activation of the apoptotic signalling enzyme caspase-9, using short DNA elements as modular recruitment domains. Caspase-9 assembly and activation on the HTHP platform due to enhanced proximity is followed by combinatorial inhibition at high scaffold concentrations. The DNA recruitment domains allow for reversible switching of the caspase-9 assembly and activity state using short modulatory DNA strands. Tuning of the recruitment domain affinity allows for generating kinetically trapped active enzyme complexes, as well as for dynamic repositioning of caspases over scaffold populations and inhibition using monovalent sink platforms. The conceptual combination of a highly structured multivalent protein platform with modular DNA recruitment domains provides emergent biomimicry properties with advanced levels of control over protein assembly.

Originele taal-2Engels
Pagina's (van-tot)11262-11266
Aantal pagina's5
TijdschriftAngewandte Chemie - International Edition
Volume60
Nummer van het tijdschrift20
Vroegere onlinedatum16 mrt 2021
DOI's
StatusGepubliceerd - 10 mei 2021

Bibliografische nota

© 2021 Wiley-VCH GmbH.

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