DNA-specific biosensors based on intramolecular β-lactamase-inhibitor complex formation

W. Engelen, M. Merkx

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukAcademicpeer review

1 Citaat (Scopus)

Samenvatting

Synthetic protein switches that sequence-specifically respond to oligonucleotide-based input triggers provide valuable tools for the readout of oligonucleotide-based biomolecular systems and networks. Here, we discuss a highly modular approach to reversibly control the DNA-directed assembly and disassembly of a complex between TEM1-β-lactamase and its inhibitor protein BLIP. By conjugating each protein to a unique handle oligonucleotide, the enzyme-inhibitor pair is noncovalently assembled upon the addition of a complementary ssDNA template strand, resulting in inhibition of enzyme activity. Hybridization of an input-oligonucleotide that is complementary to a target recognition sequence in the ssDNA template strand results in the formation of a rigid dsDNA helix that mechanically disrupts the enzyme-inhibitor complex, hereby restoring enzyme activity. Following this noncovalent approach allowed straightforward tuning of the ssDNA template recognition sequence and target oligonucleotide lengths with only a single set of oligonucleotide-functionalized enzyme and inhibitor domains. Using a fluorescent substrate, as little as 10 pM target oligonucleotide resulted in a distinguishable increase in enzyme activity.
Originele taal-2Engels
TitelSynthetic protein switches
RedacteurenV. Stein
Plaats van productieNew York
UitgeverijHumana Press
Pagina's179-194
Aantal pagina's16
ISBN van elektronische versie978-1-4939-6940-1
ISBN van geprinte versie978-1-4939-6938-8
DOI's
StatusGepubliceerd - 1 jan 2017

Publicatie series

NaamMethods in Molecular Biology
Volume1596
ISSN van geprinte versie1064-3745

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