DNA sequencing with reagent recycling on wiregrid

Lub Johan (Uitvinder), Jan Van Der Zaag Pieter (Uitvinder), Wimberger-Friedl Reinhold (Uitvinder)

Onderzoeksoutput: OctrooiOctrooi-publicatie

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Samenvatting

The present invention relates to DNA sequencing with reagent cycling on the wiregrid. The sequencing approach suggested with which allows to use a single fluid with no washing steps. Based on strong optical confinement and of excitation light and of cleavage light, the sequencing reaction can be read-out without washing the surface. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moietys. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked.

Originele taal-2Engels
OctrooinummerWO2013105025
IPCG01N 21/ 64 A I
Prioriteitsdatum9/01/13
Indieningsdatum9/01/13
StatusGepubliceerd - 13 jun. 2017

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